ArticleBMC biology2017
Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins.
Article in BMC biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- Val109 and Ile170 contribute to channel function in Guillardia theta-derived anion channelrhodopsins.Scientific reports · 2026Article
- Optogenetic silencing by combining a rhodopsin cyclase with an engineered cGMP-gated potassium channel.Science advances · 2025Article
- Twists to Classical Conditioning of AdultCold Spring Harbor protocols · 2025Article
- Modal focal adaptive optics for Bessel-focus two-photon fluorescence microscopy.Optics express · 2025Article
- Review
- Kalium channelrhodopsins effectively inhibit neurons.Nature communications · 2024Article
- Diving into the zebrafish brain: exploring neuroscience frontiers with genetic tools, imaging techniques, and behavioral insights.Frontiers in molecular neuroscience · 2024Review
- Sequential absorption of two photons creates a bistable form of RubyACR responsible for its strong desensitization.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Review
- Insights on gastrointestinal motility through the use of optogenetic sensors and actuators.The Journal of physiology · 2022Review
- Article
- Seeing the Light: The Use of Zebrafish for Optogenetic Studies of the Heart.Frontiers in physiology · 2021Review
- Multiple convergent hypothalamus-brainstem circuits drive defensive behavior.Nature neuroscience · 2020Article
- Article
- Activation of specific mushroom body output neurons inhibits proboscis extension and sucrose consumption.PloS one · 2020Article
- The Effects of Chloride Flux onMethods and protocols · 2019Article
- Quantitation of the neural silencing activity of anion channelrhodopsins in Caenorhabditis elegans and their applicability for long-term illumination.Scientific reports · 2019Article
- Article
- Potassium channel-based optogenetic silencing.Nature communications · 2018Article
- High-efficiency optogenetic silencing with soma-targeted anion-conducting channelrhodopsins.Nature communications · 2018Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOptical silencing of activity provides a way to test the necessity of neurons in behaviour. Two light-gated anion channels, GtACR1 and GtACR2, have recently been shown to potently inhibit activity in cultured mammalian neurons and in Drosophila. Here, we test the usefulness of these channels in larval zebrafish, using spontaneous coiling behaviour as the assay.
resultsWhen the GtACRs were expressed in spinal neurons of embryonic zebrafish and actuated with blue or green light, spontaneous movement was inhibited. In GtACR1-expressing fish, only 3 μW/mm
conclusionsThese results show that GtACR1 and GtACR2 can be used to optically inhibit neurons in larval zebrafish with high efficiency. The activity elicited at light offset needs to be taken into consideration in experimental design, although this property can provide insight into the effects of transiently stimulating a circuit.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.