ArticlePloS one2017
Transcriptome analysis of Phelipanche aegyptiaca seed germination mechanisms stimulated by fluridone, TIS108, and GR24.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 54 citations in OpenAlex.
- Methods of Control of Parasitic Weeds of the GenusPlants (Basel, Switzerland) · 2025Review
- Broomrapes in Major Mediterranean Crops: From Management Strategies to Novel Approaches for Next-Generation Control.Biotech (Basel (Switzerland)) · 2025Review
- Comparative transcriptome analysis provides novel insights into the seed germination of Panax japonicus, an endangered species in China.BMC plant biology · 2024Article
- Decoding the Transcriptomics of Oil Palm Seed Germination.Plants (Basel, Switzerland) · 2024Article
- Identification and expression of strigolactone biosynthesis and signaling genes and the in vitro effects of strigolactones in olive (Plant direct · 2024Article
- Comparative transcriptome analysis reveals the potential mechanism of GAFrontiers in plant science · 2024Article
- The role of small RNAs in resistant melon cultivar againstFrontiers in microbiology · 2024Article
- Phenotypic and histological analyses on the resistance of melon toFrontiers in plant science · 2023Article
- Genomic reconfiguration in parasitic plants involves considerable gene losses alongside global genome size inflation and gene births.Plant physiology · 2021Article
- Transcriptome analysis of Cinnamomum migao seed germination in medicinal plants of Southwest China.BMC plant biology · 2021Article
- Adaptation of the parasitic plant lifecycle: germination is controlled by essential host signaling molecules.Plant physiology · 2021Article
- Molecular actors of seed germination and haustoriogenesis in parasitic weeds.Plant physiology · 2021Article
- Transcriptome Analysis of Jojoba (Plants (Basel, Switzerland) · 2020Article
- Comparative transcriptome analysis revealing the potential mechanism of seed germination stimulated by exogenous gibberellin in Fraxinus hupehensis.BMC plant biology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
P. aegyptiaca is one of the most destructive root parasitic plants worldwide, causing serious damage to many crop species. Under natural conditions P. aegyptiaca seeds must be conditioned and then stimulated by host root exudates before germinating. However, preliminary experiments indicated that TIS108 (a triazole-type inhibitor of strigolactone) and fluridone (FL, an inhibitor of carotenoid-biosynthesis) both stimulated the germination of P. aegyptiaca seeds without a water preconditioning step (i.e. unconditioned seeds). The objective of this study was to use deep RNA sequencing to learn more about the mechanisms by which TIS108 and FL stimulate the germination of unconditioned P. aegyptiaca seeds. Deep RNA sequencing was performed to compare the mechanisms of germination in the following treatments: (i) unconditioned P. aegyptiaca seeds with no other treatment, (ii) unconditioned seeds treated with 100 mg/L TIS108, (iii) unconditioned seeds treated with 100 mg/L FL + 100 mg/L GA3, (iv) conditioned seeds treated with sterile water, and (v) conditioned seeds treated with 0.03 mg/L GR24. The de novo assembled transcriptome was used to analyze transcriptional dynamics during seed germination. The key gene categories involved in germination were also identified. The results showed that only 119 differentially expressed genes were identified in the conditioned treatment vs TIS108 treatment. This indicated that the vast majority of conditions for germination were met during the conditioning stage. Abscisic acid (ABA) and gibberellic acid (GA) played important roles during P. aegyptiaca germination. The common pathway of TIS108, FL+GA3, and GR24 in stimulating P. aegyptiaca germination was the simultaneous reduction in ABA concentrations and increase GA concentrations. These results could potentially aid the identification of more compounds that are capable of stimulating P. aegyptiaca germination. Some potential target sites of TIS108 were also identified in our transcriptome data. The results of this experiment suggest that TIS108 and FL+GA3 could be used to control P. aegyptiaca through suicidal germination.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.