ArticleInternational journal of molecular sciences2017
High Resolution Melting (HRM) for High-Throughput Genotyping-Limitations and Caveats in Practical Case Studies.
Article in International journal of molecular sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers, 2 of them syntheses that pooled it.
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Who cites it
72 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- High-resolution melting analysis in veterinary medicine: a systematic review of applications and diagnostic accuracy.Tropical animal health and production · 2025Pooled it
- High resolution melting assay as a reliable method for diagnosing drug-resistant TB cases: a systematic review and meta-analysis.BMC infectious diseases · 2021Pooled it
- Identification of Acantamoeba isolated from water resources located in the northwest of Iran by using the high-resolution melting analysis assay.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Comparative Evaluation of 16S rRNA Target Regions Using PCR High-Resolution Melt Curve Analysis for Differentiation of Bacteria Associated with Bovine Mastitis.Microorganisms · 2026Article
- Application of HRM analysis for specific DNA-based identification of the Hero green cultivar in Garcinia schomburgkiana.Journal, genetic engineering & biotechnology · 2026Article
- Multivalent cations stabilize DNA duplexes beyond charge neutralization.Chemical science · 2026Article
- Article
- High-resolution melting (HRM)-based genotyping of Toxoplasma gondii in meat products: comparative evaluation of ROP18, ROP5, and B1 gene markers.World journal of microbiology & biotechnology · 2026Article
- Detection of Certain Berries in Difficult Samples by Singleplex and Multiplex Real-Time PCR-HRM: A Case Study of Pitfalls.Methods and protocols · 2026Article
- The Diversity of the DNA-Binding Landscape in the DREB/ERF Family: Focusing on Reproductive Processes in Fruit Trees With Highly Heterozygous Genome.Plant biotechnology journal · 2026Article
- Rapid Authentication of Flowers ofMolecules (Basel, Switzerland) · 2026Article
- Highly sensitive PfAgo-based biosensor for the detection of mutations in the 23S rRNA gene of macrolide-resistant Mycoplasma pneumoniae.Analytical and bioanalytical chemistry · 2025Article
- A multifaceted molecular approach to surveillance of leishmaniasis: Identification of sand fly species, Leishmania parasites, and blood meal sources using high-resolution melting analysis.PLoS neglected tropical diseases · 2025Article
- The use of high-resolution melting analysis to distinguish feline enteric and feline infectious peritonitis coronaviruses.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Rapid Detection ofGenes · 2025Article
- Detection and subtyping of Blastocystis sp. in human and animal stool samples using high-resolution melting analysis.BMC infectious diseases · 2025Article
- Article
- Dissecting the genetic diversity of cultivated tomato (3 Biotech · 2024Article
- The Sweet Cherry Tree Genotype Restricts the Aggressiveness of the Wood Decay FungiMicroorganisms · 2024Article
- Comprehensive breast cancer risk analysis with whole exome sequencing and the prevalence ofBiomedical reports · 2024Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High resolution melting (HRM) is a convenient method for gene scanning as well as genotyping of individual and multiple single nucleotide polymorphisms (SNPs). This rapid, simple, closed-tube, homogenous, and cost-efficient approach has the capacity for high specificity and sensitivity, while allowing easy transition to high-throughput scale. In this paper, we provide examples from our laboratory practice of some problematic issues which can affect the performance and data analysis of HRM results, especially with regard to reference curve-based targeted genotyping. We present those examples in order of the typical experimental workflow, and discuss the crucial significance of the respective experimental errors and limitations for the quality and analysis of results. The experimental details which have a decisive impact on correct execution of a HRM genotyping experiment include type and quality of DNA source material, reproducibility of isolation method and template DNA preparation, primer and amplicon design, automation-derived preparation and pipetting inconsistencies, as well as physical limitations in melting curve distinction for alternative variants and careful selection of samples for validation by sequencing. We provide a case-by-case analysis and discussion of actual problems we encountered and solutions that should be taken into account by researchers newly attempting HRM genotyping, especially in a high-throughput setup.
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