ArticlePrion2017
Ante-mortem detection of chronic wasting disease in recto-anal mucosa-associated lymphoid tissues from elk (Cervus elaphus nelsoni) using real-time quaking-induced conversion (RT-QuIC) assay: A blinded collaborative study.
Article in Prion, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Article
- PMCA screening of retropharyngeal lymph nodes in white-tailed deer and comparisons with ELISA and IHC.Scientific reports · 2023Article
- RT-QuIC as ultrasensitive method for prion detection.Cell and tissue research · 2023Review
- Standardization of Data Analysis for RT-QuIC-Based Detection of Chronic Wasting Disease.Pathogens (Basel, Switzerland) · 2023Article
- Review
- Detection of chronic wasting disease in feces and recto-anal mucosal associated lymphoid tissues with RT-QuIC in a naturally infected farmed white-tailed deer herd.Frontiers in veterinary science · 2022Article
- The AD tau core spontaneously self-assembles and recruits full-length tau to filaments.Cell reports · 2021Article
- Blinded RT-QuIC Analysis of α-Synuclein Biomarker in Skin Tissue From Parkinson's Disease Patients.Movement disorders : official journal of the Movement Disorder Society · 2020Article
- An Ex Vivo Brain Slice Culture Model of Chronic Wasting Disease: Implications for Disease Pathogenesis and Therapeutic Development.Scientific reports · 2020Article
- Detection of Pathognomonic Biomarker PrPBiomolecules · 2020Review
- Article
- α-Synuclein real-time quaking-induced conversion in the submandibular glands of Parkinson's disease patients.Movement disorders : official journal of the Movement Disorder Society · 2020Article
- Evaluation of Antemortem Diagnostic Techniques in Goats Naturally Infected With Scrapie.Frontiers in veterinary science · 2020Article
- Progression of chronic wasting disease in white-tailed deer analyzed by serial biopsy RT-QuIC and immunohistochemistry.PloS one · 2020Article
- Use of different RT-QuIC substrates for detecting CWD prions in the brain of Norwegian cervids.Scientific reports · 2019Article
- Ultrasensitive Detection of Aggregated α-Synuclein in Glial Cells, Human Cerebrospinal Fluid, and Brain Tissue Using the RT-QuIC Assay: New High-Throughput Neuroimmune Biomarker Assay for Parkinsonian Disorders.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2019Article
- Comparison of conventional, amplification and bio-assay detection methods for a chronic wasting disease inoculum pool.PloS one · 2019Article
- Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Prion diseases are transmissible spongiform encephalopathies (TSEs) characterized by fatal, progressive neurologic diseases with prolonged incubation periods and an accumulation of infectious misfolded prion proteins. Antemortem diagnosis is often difficult due to a long asymptomatic incubation period, differences in the pathogenesis of different prions, and the presence of very low levels of infectious prion in easily accessible samples. Chronic wasting disease (CWD) is a TSE affecting both wild and captive populations of cervids, including mule deer, white-tailed deer, elk, moose, muntjac, and most recently, wild reindeer. This study represents a well-controlled evaluation of a newly developed real-time quaking-induced conversion (RT-QuIC) assay as a potential CWD diagnostic screening test using rectal biopsy sections from a depopulated elk herd. We evaluated 69 blinded samples of recto-anal mucosa-associated lymphoid tissue (RAMALT) obtained from USDA Veterinary Services. The results were later un-blinded and statistically compared to immunohistochemical (IHC) results from the USDA National Veterinary Services Laboratories (NVSL) for RAMALT, obex, and medial retropharyngeal lymph node (MRPLN). Comparison of RAMALT RT-QuIC assay results with the IHC results of RAMALT revealed 92% relative sensitivity (95% confidence limits: 61.52-99.8%) and 95% relative specificity (95% confidence limits: 85.13-99%). Collectively, our results show a potential utility of the RT-QuIC assay to advance the development of a rapid, sensitive, and specific prion diagnostic assay for CWD prions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.