ArticleAMB Express2017
Linear and exponential TAIL-PCR: a method for efficient and quick amplification of flanking sequences adjacent to Tn5 transposon insertion sites.
Article in AMB Express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
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- Transgene Mapping in Animals: What to Choose?International journal of molecular sciences · 2025Review
- Conserved immunomodulation and variation in host association by Xanthomonadales commensals in Arabidopsis root microbiota.Nature plants · 2025Article
- Primer extension refractory PCR: an efficient and reliable genome walking method.Molecular genetics and genomics : MGG · 2024Article
- Semi-Site-Specific Primer PCR: A Simple but Reliable Genome-Walking Tool.Current issues in molecular biology · 2023Article
- Bridging PCR: An Efficient and Reliable Scheme Implemented for Genome-Walking.Current issues in molecular biology · 2023Article
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- Wristwatch PCR: A Versatile and Efficient Genome Walking Strategy.Frontiers in bioengineering and biotechnology · 2022Article
- Editorial: Innovative applications of sequencing technologies in plant science.Frontiers in plant science · 2022Article
- Fusion primer driven racket PCR: A novel tool for genome walking.Frontiers in genetics · 2022Article
- Meningitic Escherichia coli α-hemolysin aggravates blood-brain barrier disruption via targeting TGFβ1-triggered hedgehog signaling.Molecular brain · 2021Article
- Expression and Refolding of the Plant Chitinase FromFrontiers in bioengineering and biotechnology · 2021Article
- Identification of Essential Genes Associated With Prodigiosin Production inFrontiers in microbiology · 2021Article
- Simple innovative adaptor to improve genome walking with convenient PCR.Journal, genetic engineering & biotechnology · 2020Article
- Cyclic Digestion and Ligation-Mediated PCR Used for Flanking Sequence Walking.Scientific reports · 2020Article
- Disrupting MLV integrase:BET protein interaction biases integration into quiescent chromatin and delays but does not eliminate tumor activation in a MYC/Runx2 mouse model.PLoS pathogens · 2019Article
- Palindromic sequence-targeted (PST) PCR: a rapid and efficient method for high-throughput gene characterization and genome walking.Scientific reports · 2019Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current genome walking methods are very time consuming, and many produce non-specific amplification products. To amplify the flanking sequences that are adjacent to Tn5 transposon insertion sites in Serratia marcescens FZSF02, we developed a genome walking method based on TAIL-PCR. This PCR method added a 20-cycle linear amplification step before the exponential amplification step to increase the concentration of the target sequences. Products of the linear amplification and the exponential amplification were diluted 100-fold to decrease the concentration of the templates that cause non-specific amplification. Fast DNA polymerase with a high extension speed was used in this method, and an amplification program was used to rapidly amplify long specific sequences. With this linear and exponential TAIL-PCR (LETAIL-PCR), we successfully obtained products larger than 2 kb from Tn5 transposon insertion mutant strains within 3 h. This method can be widely used in genome walking studies to amplify unknown sequences that are adjacent to known sequences.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.