Evidence map›Paper›PMID 29098316›Full record

ArticleJournal of molecular medicine (Berlin, Germany)2018

Plasma circular RNA profiling of patients with gastric cancer and their droplet digital RT-PCR detection.

Tianwen Li, Yongfu Shao, Liyun Fu, Yi Xie, Linwen Zhu, Weiliang Sun, Rui Yu, Bingxiu Xiao, Junming Guo

Open access · hybridAbstract read
PubMed Publisher
In one paragraph

Article in Journal of molecular medicine (Berlin, Germany), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 153 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
153citing papers in PubMed, 4 pooled it
13.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

153 citing papers in PubMed, 4 syntheses or guidelines pooled it, 271 citations in OpenAlex.

  1. Pooled it
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  13. Roles and Applications of Circular RNA in Virus Infection.International journal of molecular sciences · 2025
    Review
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  19. Article
  20. World journal of gastrointestinal oncology · 2025
    Review

93 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Tianwen LiDepartment of Biochemistry and Molecular Biology, and Zhejiang Key Laboratory of Pathophysiology, Medical School, Ningbo University, Ningbo, 315211, China.
Yongfu ShaoDepartment of Gastroenterology, the Affiliated Hospital of Medical School of, Ningbo University, Ningbo, 315020, China.
Liyun FuDepartment of Hepatology, Ningbo No. 2 Hospital and the Affiliated Hospital, Medical School, Ningbo University, Ningbo, 315010, China.
Yi XieDepartment of Biochemistry and Molecular Biology, and Zhejiang Key Laboratory of Pathophysiology, Medical School, Ningbo University, Ningbo, 315211, China.
Linwen ZhuDepartment of Biochemistry and Molecular Biology, and Zhejiang Key Laboratory of Pathophysiology, Medical School, Ningbo University, Ningbo, 315211, China.
Weiliang SunNingbo Yinzhou People's Hospital and the Affiliated Hospital, Medical School, Ningbo University, Ningbo, 315040, China.
Rui YuDepartment of Biochemistry and Molecular Biology, and Zhejiang Key Laboratory of Pathophysiology, Medical School, Ningbo University, Ningbo, 315211, China.
Bingxiu XiaoDepartment of Biochemistry and Molecular Biology, and Zhejiang Key Laboratory of Pathophysiology, Medical School, Ningbo University, Ningbo, 315211, China.
Junming GuoDepartment of Biochemistry and Molecular Biology, and Zhejiang Key Laboratory of Pathophysiology, Medical School, Ningbo University, Ningbo, 315211, China. guojunming@nbu.edu.cn.ORCID 0000-0003-2026-1075
Ningbo University · CN

Funding

Applied Research Project on Nonprofit Technology of Zhejiang Province 2016C33177National Natural Science Foundation of China 81772279Scientific Innovation Team Project of Ningbo 2017B00001
6 · The paper itself

Abstract

To observe the diagnostic values of circular RNAs (circRNAs), their expression profiles between gastric cancer patients' plasma and healthy controls were first assessed by circRNA microarray. Then, circRNA levels were measured by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and RT-droplet digital PCR (RT-ddPCR), respectively. A total of 343 differentially expressed circRNAs were found. The top 10 elevated circRNAs in patients were hsa_circ_0088300, hsa_circ_0075825, hsa_circ_0019172, hsa_circ_0000220, hsa_circ_0035277, hsa_circ_0000301, hsa_circ_0000189, hsa_circ_0090080, hsa_circ_0001888, and hsa_circ_0001874. The top 10 reduced circRNAs were hsa_circ_0004771, hsa_circ_0001190, hsa_circ_0061276, hsa_circ_0092337, hsa_circ_0058495, hsa_circ_0061274, hsa_circ_0075829, hsa_circ_0080845, hsa_circ_0001006, and hsa_circ_0003764. In cancer and dysplasia tissues, hsa_circ_0001017 and hsa_circ_0061276 were downregulated. Their levels were significantly associated with distal metastasis. The area under receiver operating characteristic curve in combinative use was 0.966 with 95.5% sensitivity and 95.7% specificity. Patients with low plasma hsa_circ_0001017 or hsa_circ_0061276 had a much shorter overall survival than those with high levels. Patients whose plasma hsa_circ_0001017 or hsa_circ_0061276 levels recovered to normal after operation had a longer disease-free survival. Finally, the in vitro model indicated gastric cancer cells secreting circRNAs into plasma. In conclusion, RT-ddPCR is a potent non-invasive and absolute quantification method for simultaneous detection of multiple circRNAs. Hsa_circ_0001017 and hsa_circ_0061276 are new potential biomarkers for gastric cancer. KEY MESSAGE: A total of 343 circRNAs are differentially expressed between gastric cancer patients' plasma and healthy controls. Hsa_circ_0001017 and hsa_circ_0061276 are downregulated in gastric cancer tissues. The RT-ddPCR is a potent method for simultaneous detection of multiple circRNAs in plasma. Hsa_circ_0001017 and hsa_circ_0061276 are potential biomarkers for gastric cancer.

Indexed as

Biomarkers, TumorCell Line, TumorFemaleHumansMaleMiddle AgedReverse Transcriptase Polymerase Chain ReactionRNARNA, CircularStomach NeoplasmsBiomarkers, TumorRNARNA, CircularBiomarkerDigital polymerase chain reactionGastric cancerNon-invasive testing

Identifiers

PMID29098316
OpenAlexW2766204235

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.