ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2018
A single nucleotide polymorphism in the "Fra" gene results in fractured starch granules in barley.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Regulatory factors of starch metabolism in cereal endosperm.Plant molecular biology · 2026Review
- Modification of starch synthesis in food crops using CRISPR/Cas9 gene editing technology for changing climate.Stress biology · 2026Review
- ZmSSRP1, transactivated by OPAQUE11, positively regulates starch biosynthesis in maize endosperm.Plant biotechnology journal · 2025Article
- Map-based cloning of ZmSS5, construction and validation of its regulatory pathway for maize kernel weight.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Development of a KASP marker set for high-throughput genotyping in Japanese barley breeding programs with various end-use purposes.Breeding science · 2025Article
- Distinct effects of PTST2b and MRC on starch granule morphogenesis in potato tubers.Plant biotechnology journal · 2025Article
- Wheat MYOSIN-RESEMBLING CHLOROPLAST PROTEIN controls B-type starch granule initiation timing during endosperm development.Plant physiology · 2024Article
- Mutations in starch BRANCHING ENZYME 2a suppress the traits caused by the loss of ISOAMYLASE1 in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Customizing Starch Properties: A Review of Starch Modifications and Their Applications.Polymers · 2023Review
- FLOURY ENDOSPERM 6 mutations enhance the sugary phenotype caused by the loss of ISOAMYLASE1 in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Loss of PROTEIN TARGETING TO STARCH 2 has variable effects on starch synthesis across organs and species.Journal of experimental botany · 2022Article
- Developing core marker sets for effective genomic-assisted selection in wheat and barley breeding programs.Breeding science · 2022Article
- Starch biosynthesis in cereal endosperms: An updated review over the last decade.Plant communications · 2021Review
- STARCH SYNTHASE5, a Noncanonical Starch Synthase-Like Protein, Promotes Starch Granule Initiation in Arabidopsis.The Plant cell · 2020Article
- The A to B of starch granule formation in wheat endosperm.Journal of experimental botany · 2020Article
- Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation.Journal of experimental botany · 2020Article
- Protein Targeting to Starch 1 is essential for starchy endosperm development in barley.Journal of experimental botany · 2019Article
- Two Plastidial Coiled-Coil Proteins Are Essential for Normal Starch Granule Initiation in Arabidopsis.The Plant cell · 2018Article
- Functional Roles of Starch Binding Domains and Surface Binding Sites in Enzymes Involved in Starch Biosynthesis.Frontiers in plant science · 2018Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageWe report here that the mutation causing fractured starch granules in the barley line "Franubet" results from a point mutation in the barley gene corresponding to the rice FLO6 gene. The "fra" mutation in barley, which was originally isolated and characterized over 30 years ago, results in fractured starch granules and an opaque phenotype. This mutation has been used in breeding programs, since it appears to be useful in the production of pearled barley for human consumption. However, selection for this phenotype is difficult, since wild-type and heterozygous kernels cannot be distinguished phenotypically, and until now, the gene involved in this mutation has not been determined. Here, we used a map-based cloning approach using nanopore sequencing to obtain long reads from a BAC clone carrying markers on either side of the fra locus. By fine mapping followed by aligning RNA-seq reads to four genes within the mapped region, we were able to determine that the fra mutation is caused by the introduction of a stop codon in the barley homologue of the rice FLOURY ENDOSPERM 6 (FLO6) gene. This gene has a CBM48 domain that binds to starch, and may act through interactions with isoamylase1 (ISA1), assisting in the binding of ISA1 to starch granules. Perfect markers able to distinguish all genotypes were designed and tested in several large populations; in all cases, the markers were able to distinguish wild-type, heterozygous, and mutant genotypes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.