ArticleScientific reports2017
Freeform micropatterning of living cells into cell culture medium using direct inkjet printing.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 101 citations in OpenAlex.
- A Single-Cell Bioprinting Method to Reconstruct Native Cellular Microenvironments with Subcellular Resolution.Research square · 2026Article
- Functional Nano-to-Microstructures by Jet Printing and Direct Ink Writing.Chemical reviews · 2026Review
- Bioinks and biofabrication techniques for biosensors development: A review.Materials today. Bio · 2024Review
- Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications.Advanced drug delivery reviews · 2024Review
- Multiplex Single-Cell Bioprinting for Engineering of Heterogeneous Tissue Constructs with Subcellular Spatial Resolution.bioRxiv : the preprint server for biology · 2024Article
- Review
- Keeping It Organized: Multicompartment Constructs to Mimic Tissue Heterogeneity.Advanced healthcare materials · 2023Review
- Article
- Understanding droplet jetting on varying substrate for biological applications.International journal of bioprinting · 2023Review
- Scalable fabrication, compartmentalization and applications of living microtissues.Bioactive materials · 2023Article
- Article
- All-Inkjet-Printed 3D Alveolar Barrier Model with Physiologically Relevant Microarchitecture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Engineered Tools to Study Intercellular Communication.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- High-Definition Single-Cell Printing: Cell-by-Cell Fabrication of Biological Structures.Advanced materials (Deerfield Beach, Fla.) · 2020Article
- Photo-Cleavable Peptide-Poly(Ethylene Glycol) Conjugate Surfaces for Light-Guided Control of Cell Adhesion.Micromachines · 2020Article
- Aqueous Processed Biopolymer Interfaces for Single-Cell Microarrays.ACS biomaterials science & engineering · 2020Article
- Recapitulating complex biological signaling environments using a multiplexed, DNA-patterning approach.Science advances · 2020Article
- 3D Printed Neural Regeneration Devices.Advanced functional materials · 2020Article
- Stereolithography 3D Bioprinting.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Engineering a Model to Study Viral Infections: Bioprinting, Microfluidics, and Organoids to Defeat Coronavirus Disease 2019 (COVID-19).International journal of bioprinting · 2020Review
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microfabrication methods have widely been used to control the local cellular environment on a micron scale. However, accurately mimicking the complexity of the in vivo tissue architecture while maintaining the freedom of form and design is still a challenge when co-culturing multiple types of cells on the same substrate. For the first time, we present a drop-on-demand inkjet printing method to directly pattern living cells into a cell-friendly liquid environment. High-resolution control of cell location is achieved by precisely optimizing printing parameters with high-speed imaging of cell jetting and impacting behaviors. We demonstrated the capabilities of the direct cell printing method by co-printing different cells into various designs, including complex gradient arrangements. Finally, we applied this technique to investigate the influence of the heterogeneity and geometry of the cell population on the infectivity of seasonal H1N1 influenza virus (PR8) by generating A549 and HeLa cells printed in checkboard patterns of different sizes in a medium-filled culture dish. Direct inkjet cell patterning can be a powerful and versatile tool for both fundamental biology and applied biotechnology.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.