ArticleScientific reports2017
Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Small RNA Sequencing Reveals Dynamic Expression Profiles and Regulatory Network of miRNAs During Testis Development of Macrobrachium rosenbergii.Marine biotechnology (New York, N.Y.) · 2026Article
- HP1-Mediated Silencing of theJournal of developmental biology · 2025Article
- Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna.Scientific reports · 2024Article
- Identification of gene isoforms and their switching events between male and female embryos of the parthenogenetic crustacean Daphnia magna.Scientific reports · 2024Article
- CELF1 represses Doublesex1 expression via its 5' UTR in the crustacean Daphnia magna.PloS one · 2022Article
- Regulation of Doublesex1 Expression for Environmental Sex Determination in the Cladoceran Crustacean Daphnia.Frontiers in cell and developmental biology · 2022Review
- Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression.PLoS genetics · 2021Article
- A Crab Is Not a Fish: Unique Aspects of the Crustacean Endocrine System and Considerations for Endocrine Toxicology.Frontiers in endocrinology · 2021Review
- A comprehensive epigenomic analysis of phenotypically distinguishable, genetically identical female and male Daphnia pulex.BMC genomics · 2020Article
- Two Doublesex1 mutants revealed a tunable gene network underlying intersexuality in Daphnia magna.PloS one · 2020Article
- Phylogenetic analysis and embryonic expression of panarthropod Dmrt genes.Frontiers in zoology · 2019Article
- Sensory Ecology of Predator-Induced Phenotypic Plasticity.Frontiers in behavioral neuroscience · 2018Review
- Article
- Co-option of the bZIP transcription factor Vrille as the activator of Doublesex1 in environmental sex determination of the crustacean Daphnia magna.PLoS genetics · 2017Article
Corrections and comments
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Sexually dimorphic traits are common and widespread among animals. The expression of the Doublesex-/Mab-3-domain (DM-domain) gene family has been widely studied in model organisms and has been proven to be essential for the development and maintenance of sex-specific traits. However, little is known about the detailed expression patterns in non-model organisms. In the present study, we demonstrated the spatiotemporal expression of the DM-domain gene, doublesex1 (dsx1), in the crustacean Daphnia magna, which parthenogenetically produces males in response to environmental cues. We developed a dsx1 reporter strain to track dsx1 activity in vivo by inserting the mCherry gene into the dsx1 locus using the TALEN-mediated knock-in approach. After confirming dsx1 expression in male-specific traits in juveniles and adults, we performed time-lapse imaging of embryogenesis. Shortly after gastrulation stage, a presumptive primary organiser, named cumulus, first showed male-specific dsx1 expression. This cell mass moved to the posterior growth zone that distributes dsx1-expressing progenitor cells across the body during axial elongation, before embryos start male-specific dsx1 expression in sexually dimorphic structures. The present study demonstrated the sex-specific dsx1 expression in cell populations involved in basal body formation.
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