ArticleNature communications2017
Genome-scale single-cell mechanical phenotyping reveals disease-related genes involved in mitotic rounding.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Reconstitution of actomyosin networks in cell-sized liposomes dissects distinct mechanisms of membrane blebbing and symmetry breaking.Science advances · 2026Article
- High-throughput mechanomic screening reveals novel regulators of single-cell mechanics.Biophysical journal · 2026Article
- TMEM63B regulates nucleocytoplasmic transport and placental development.Nature communications · 2026Article
- CDK1-mediated phosphorylation of EPLIN is crucial for mitotic spindle positioning.Communications biology · 2026Article
- Reconstitution of actomyosin networks in cell-sized liposomes reveals distinct mechanical roles of cytoskeletal organization in membrane shape remodeling.bioRxiv : the preprint server for biology · 2025Article
- Interphase cell morphology defines the mode, symmetry, and outcome of mitosis.Science (New York, N.Y.) · 2025Article
- Article
- The Role of the Swollen State in Cell Proliferation.The Journal of membrane biology · 2025Review
- YAP Overcomes Mechanical Barriers to Induce Mitotic Rounding and Adult Cardiomyocyte Division.Circulation · 2025Article
- Signaling Regulation of FAM134-Dependent ER-Phagy in Cells.Journal of cellular physiology · 2025Review
- Mechanosensitive accumulation of non-muscle myosin IIB during mitosis requires its translocation activity.iScience · 2023Article
- Microtubules oppose cortical actomyosin-driven membrane ingression during C. elegans meiosis I polar body extrusion.PLoS genetics · 2023Article
- Reduction of Intracellular Tension and Cell Adhesion Promotes Open Chromatin Structure and Enhances Cell Reprogramming.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Cortical microtubules oppose actomyosin-driven membrane ingression duringbioRxiv : the preprint server for biology · 2023Article
- Dynamics of cell rounding during detachment.iScience · 2023Article
- Article
- Background and roles: myosin in autoimmune diseases.Frontiers in cell and developmental biology · 2023Review
- Proteomic analysis of the actin cortex in interphase and mitosis.Journal of cell science · 2022Article
- Interphase microtubule disassembly is a signaling cue that drives cell rounding at mitotic entry.The Journal of cell biology · 2022Article
- Protein and lipid mass concentration measurement in tissues by stimulated Raman scattering microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To divide, most animal cells drastically change shape and round up against extracellular confinement. Mitotic cells facilitate this process by generating intracellular pressure, which the contractile actomyosin cortex directs into shape. Here, we introduce a genome-scale microcantilever- and RNAi-based approach to phenotype the contribution of > 1000 genes to the rounding of single mitotic cells against confinement. Our screen analyzes the rounding force, pressure and volume of mitotic cells and localizes selected proteins. We identify 49 genes relevant for mitotic rounding, a large portion of which have not previously been linked to mitosis or cell mechanics. Among these, depleting the endoplasmic reticulum-localized protein FAM134A impairs mitotic progression by affecting metaphase plate alignment and pressure generation by delocalizing cortical myosin II. Furthermore, silencing the DJ-1 gene uncovers a link between mitochondria-associated Parkinson's disease and mitotic pressure. We conclude that mechanical phenotyping is a powerful approach to study the mechanisms governing cell shape.
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