ArticleScientific reports2017
Anion-conducting channelrhodopsins with tuned spectra and modified kinetics engineered for optogenetic manipulation of behavior.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed.
- Val109 and Ile170 contribute to channel function in Guillardia theta-derived anion channelrhodopsins.Scientific reports · 2026Article
- Effects of corticothalamic feedback depend on visual responsiveness and stimulus type.iScience · 2025Article
- Review
- Article
- Chloride-dependent mechanisms of multimodal sensory discrimination and nociceptive sensitization ineLife · 2023Article
- Practical considerations in an era of multicolor optogenetics.Frontiers in cellular neuroscience · 2023Article
- Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles.Nature communications · 2022Article
- Aion is a bistable anion-conducting channelrhodopsin that provides temporally extended and reversible neuronal silencing.Communications biology · 2022Article
- Review
- Engineering rhodopsins' activation spectra using a FRET-based approach.Biophysical journal · 2022Article
- Selective optogenetic control of GNature communications · 2022Article
- Advancements in the Quest to Map, Monitor, and Manipulate Neural Circuitry.Frontiers in neural circuits · 2022Review
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- Article
- BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons.Nature communications · 2021Article
- A synthetic circuit for buffering gene dosage variation between individual mammalian cells.Nature communications · 2021Article
- Preference of Proteomonas sulcata anion channelrhodopsin for NOScientific reports · 2021Article
- Membrane potential regulates Hedgehog signalling in the Drosophila wing imaginal disc.EMBO reports · 2021Article
- Specific residues in the cytoplasmic domain modulate photocurrent kinetics of channelrhodopsin from Klebsormidium nitens.Communications biology · 2021Article
- Structure-Function Relationship of Channelrhodopsins.Advances in experimental medicine and biology · 2021Review
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
Abstract
Genetic engineering of natural light-gated ion channels has proven a powerful way to generate optogenetic tools for a wide variety of applications. In recent years, blue-light activated engineered anion-conducting channelrhodopsins (eACRs) have been developed, improved, and were successfully applied in vivo. We asked whether the approaches used to create eACRs can be transferred to other well-characterized cation-conducting channelrhodopsins (CCRs) to obtain eACRs with a broad spectrum of biophysical properties. We generated 22 variants using two conversion strategies applied to 11 CCRs and screened them for membrane expression, photocurrents and anion selectivity. We obtained two novel eACRs, Phobos and Aurora, with blue- and red-shifted action spectra and photocurrents similar to existing eACRs. Furthermore, step-function mutations greatly enhanced the cellular operational light sensitivity due to a slowed-down photocycle. These bi-stable eACRs can be reversibly toggled between open and closed states with brief light pulses of different wavelengths. All new eACRs reliably inhibited action potential firing in pyramidal CA1 neurons. In Drosophila larvae, eACRs conveyed robust and specific light-dependent inhibition of locomotion and nociception.
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Registered trials
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