ArticleScientific reports2017
Translation efficiency is a determinant of the magnitude of miRNA-mediated repression.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Multi-dimensional regulation of LIN-28 temporal expression dynamics in the C. elegans heterochronic gene cascade.Development (Cambridge, England) · 2026Article
- Multi-Tissue Omics Analysis Uncovers Translational Regulation Underlying Complex Traits in Pigs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Biochemical principles of miRNA targeting in flies.Nature communications · 2026Article
- Approaches for identification of 5' UTR mutations impacting translation and protein production from neurodevelopmental disorder genes.Cell reports methods · 2025Article
- Effects of Dietary Fiber, Phenolic Compounds, and Fatty Acids on Mental Health: Possible Interactions with Genetic and Epigenetic Aspects.Nutrients · 2024Review
- A Massively Parallel Screen of 5'UTR Mutations Identifies Variants Impacting Translation and Protein Production in Neurodevelopmental Disorder Genes.medRxiv : the preprint server for health sciences · 2023Article
- Single-molecule imaging reveals translation-dependent destabilization of mRNAs.Molecular cell · 2023Article
- Article
- Implications of tRNA abundance on translation elongation across bovine tissues.Frontiers in genetics · 2023Article
- Binding sites of miRNA on the overexpressed genes of oral cancer using 7mer-seed match.Molecular and cellular biochemistry · 2022Article
- Codon optimality-mediated mRNA degradation: Linking translational elongation to mRNA stability.Molecular cell · 2022Review
- Context-aware synthetic biology by controller design: Engineering the mammalian cell.Cell systems · 2021Review
- Article
- Upregulation of 5'-terminal oligopyrimidine mRNA translation upon loss of the ARF tumor suppressor.Scientific reports · 2020Article
- Extracellular Vesicles Derived Human-miRNAs Modulate the Immune System in Type 1 Diabetes.Frontiers in cell and developmental biology · 2020Article
- Coding regions affect mRNA stability in human cells.RNA (New York, N.Y.) · 2019Article
- Quantitative principles of cis-translational control by general mRNA sequence features in eukaryotes.Genome biology · 2019Article
- Quantification and discovery of sequence determinants of protein-per-mRNA amount in 29 human tissues.Molecular systems biology · 2019Article
- Temporospatial guidance of activity-dependent gene expression by microRNA: mechanisms and functional implications for neural plasticity.Nucleic acids research · 2019Review
- MicroRNA-668 represses MTP18 to preserve mitochondrial dynamics in ischemic acute kidney injury.The Journal of clinical investigation · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
MicroRNAs are well known regulators of mRNA stability and translation. However, the magnitude of both translational repression and mRNA decay induced by miRNA binding varies greatly between miRNA targets. This can be the result of cis and trans factors that affect miRNA binding or action. We set out to address this issue by studying how various mRNA characteristics affect miRNA-mediated repression. Using a dual luciferase reporter system, we systematically analyzed the ability of selected mRNA elements to modulate miRNA-mediated repression. We found that changing the 3'UTR of a miRNA-targeted reporter modulates translational repression by affecting the translation efficiency. This 3'UTR dependent modulation can be further altered by changing the codon-optimality or 5'UTR of the luciferase reporter. We observed maximal repression with intermediate codon optimality and weak repression with very high or low codon optimality. Analysis of ribosome profiling and RNA-seq data for endogenous miRNA targets revealed translation efficiency as a key determinant of the magnitude of miRNA-mediated translational repression. Messages with high translation efficiency were more robustly repressed. Together our results reveal modulation of miRNA-mediated repression by characteristics and features of the 5'UTR, CDS and 3'UTR.
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