Evidence map›Paper›PMID 29097366›Full record

ArticleArteriosclerosis, thrombosis, and vascular biology2018

Quantitative Trait Locus Mapping of Macrophage Cholesterol Metabolism and CRISPR/Cas9 Editing Implicate an ACAT1 Truncation as a Causal Modifier Variant.

Qimin Hai, Brian Ritchey, Peggy Robinet, Alexander M Alzayed, Greg Brubaker, Jinying Zhang, Jonathan D Smith

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arteriosclerosis, thrombosis, and vascular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 30 citations in OpenAlex.

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  15. Annual Report on Sex in Preclinical Studies:Arteriosclerosis, thrombosis, and vascular biology · 2020
    Review
  16. Functional Genomics and CRISPR Applied to Cardiovascular Research and Medicine.Arteriosclerosis, thrombosis, and vascular biology · 2019
    Article
  17. Article
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Qimin HaiFrom the Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Henan, China (Q.H., J.Z.); Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland, OH (Q.H., B.R., P.R., A.M.A., G.B., J.D.S); and Department of Chemistry, Cleveland State University, OH (B.R.).
Brian RitcheyFrom the Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Henan, China (Q.H., J.Z.); Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland, OH (Q.H., B.R., P.R., A.M.A., G.B., J.D.S); and Department of Chemistry, Cleveland State University, OH (B.R.).
Peggy RobinetFrom the Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Henan, China (Q.H., J.Z.); Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland, OH (Q.H., B.R., P.R., A.M.A., G.B., J.D.S); and Department of Chemistry, Cleveland State University, OH (B.R.).
Alexander M AlzayedFrom the Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Henan, China (Q.H., J.Z.); Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland, OH (Q.H., B.R., P.R., A.M.A., G.B., J.D.S); and Department of Chemistry, Cleveland State University, OH (B.R.).
Greg BrubakerFrom the Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Henan, China (Q.H., J.Z.); Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland, OH (Q.H., B.R., P.R., A.M.A., G.B., J.D.S); and Department of Chemistry, Cleveland State University, OH (B.R.).
Jinying ZhangFrom the Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Henan, China (Q.H., J.Z.); Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland, OH (Q.H., B.R., P.R., A.M.A., G.B., J.D.S); and Department of Chemistry, Cleveland State University, OH (B.R.). smithj4@ccf.org jyzhang@zzu.edu.cn.
Jonathan D SmithFrom the Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Henan, China (Q.H., J.Z.); Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland, OH (Q.H., B.R., P.R., A.M.A., G.B., J.D.S); and Department of Chemistry, Cleveland State University, OH (B.R.). smithj4@ccf.org jyzhang@zzu.edu.cn.
First Affiliated Hospital of Zhengzhou University · CN

Funding

Vascular Cell function and AtherosclerosisP01HL029582 · NHLBI · CLEVELAND CLINIC LERNER COM-CWRU · PI FOX, PAUL L · 1985 to 2019
$32.0M
Characterization of Atherosclerosis Modifier GenesR01HL098193 · NHLBI · CLEVELAND CLINIC LERNER COM-CWRU · PI SMITH, JONATHAN D · 2010 to 2013
$1.8M
NHLBI NIH HHS P01 HL029582NHLBI NIH HHS R01 HL098193
6 · The paper itself

Abstract

objectiveCholesterol metabolism is a dynamic process involving intracellular trafficking, cholesterol esterification, and cholesterol ester hydrolysis. Our objective was to identify genes that regulate macrophage cholesterol metabolism. APPROACHES AND

resultsWe performed quantitative trait loci mapping of free and esterified cholesterol levels and the ratio of esterified to free cholesterol in acetylated low-density lipoprotein-loaded bone marrow-derived macrophages from an AKR×DBA/2 strain intercross. Ten distinct cholesterol modifier loci were identified, and bioinformatics was used to prioritize candidate genes. The strongest locus was located on distal chromosome 1, which we named

conclusionsA Soat1 deletion present in AKR mice, and resultant N-terminal ACAT1 truncation, was confirmed to be a significant modifier of macrophage cholesterol metabolism. Other Mcmm loci candidate genes were prioritized via bioinformatics.

Indexed as

Clustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsGenes, ModifierQuantitative Trait LociAcetyl-CoA C-AcetyltransferaseAnimalsCholesterolComputational BiologyCRISPR-Associated Protein 9Crosses, GeneticGene EditingGenetic Association StudiesGenotypeMacrophagesMice, Inbred AKRMice, Inbred DBAAcat1 protein, mouseAcetyl-CoA C-AcetyltransferaseCholesterolCRISPR-Associated Protein 9Sterol O-Acyltransferasesterol O-acyltransferase 1cholesterolesterificationfoam cellsmacrophagemetabolism

Identifiers

PMID29097366
PMCPMC5746475
OpenAlexW2766072055

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.