ArticleMicrobial cell factories2017
Biosensor-aided high-throughput screening of hyper-producing cells for malonyl-CoA-derived products.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 28 citations in OpenAlex.
- Characterizing and Engineering a Succinate-Responsive Biosensor System inACS synthetic biology · 2025Article
- Engineering genetic circuits for dynamic control of central metabolism.Metabolic engineering · 2025Article
- Engineering controllable alteration of malonyl-CoA levels to enhance polyketide production.Nature chemical biology · 2025Article
- A genetically encoded fluorescent biosensor for visualization of acetyl-CoA in live cells.Cell chemical biology · 2025Article
- Transcription factor-based biosensors for screening and dynamic regulation.Frontiers in bioengineering and biotechnology · 2023Review
- Integration of a multi-step heterologous pathway in Saccharomyces cerevisiae for the production of abscisic acid.Microbial cell factories · 2019Article
- Engineering Saccharomyces cerevisiae cells for production of fatty acid-derived biofuels and chemicals.Open biology · 2019Review
- Repurposing type III polyketide synthase as a malonyl-CoA biosensor for metabolic engineering in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Engineering Escherichia coli to increase triacetic acid lactone (TAL) production using an optimized TAL sensor-reporter system.Journal of industrial microbiology & biotechnology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMalonyl-coenzyme A (CoA) is an important biosynthetic precursor in vivo. Although Escherichia coli is a useful organism for biosynthetic applications, its malonyl-CoA level is too low.
resultsTo identify strains with the best potential for enhanced malonyl-CoA production, we developed a whole-cell biosensor for rapidly reporting intracellular malonyl-CoA concentrations. The biosensor was successfully applied as a high-throughput screening tool for identifying targets at a genome-wide scale that could be critical for improving the malonyl-CoA biosynthesis in vivo. The mutant strains selected synthesized significantly higher titers of the type III polyketide triacetic acid lactone (TAL), phloroglucinol, and free fatty acids compared to the wild-type strain, using malonyl-CoA as a precursor.
conclusionThese results validated this novel whole-cell biosensor as a rapid and sensitive malonyl-CoA high-throughput screening tool. Further analysis of the mutant strains showed that the iron ion concentration is closely related to the intracellular malonyl-CoA biosynthesis.
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