Evidence map›Paper›PMID 29096322›Full record

ArticleRedox biology2018

Mechanically induced autophagy is associated with ATP metabolism and cellular viability in osteocytes in vitro.

Bingbing Zhang, Rutao Hou, Zhen Zou, Tiantian Luo, Yang Zhang, Liyun Wang, Bin Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Redox biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
5.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 72 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Bingbing ZhangKey Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
Rutao HouKey Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
Zhen ZouDepartment of Medical Laboratory Technology, Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, China.
Tiantian LuoKey Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
Yang ZhangKey Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
Liyun WangDepartment of Mechanical Engineering, University of Delaware, Newark, DE 19716, USA.
Bin WangDepartment of Medical Laboratory Technology, Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, China. Electronic address: bwang@cqmu.edu.cn.
Chongqing University · CNChongqing Medical University · CNUniversity of Delaware · US

Funding

Pilot Project ProgramP30GM103333 · NIGMS · UNIVERSITY OF DELAWARE · PI BUCHANAN, THOMAS S · 2012 to 2016
$5.7M
Supplement to R01 Titled: Mechanosensing in the Bone Lacunar-Canalicular SystemR01AR054385 · NIAMS · UNIVERSITY OF DELAWARE · PI FARACH-CARSON, MARY C, WANG, LIYUN · 2007 to 2019
$3.8M
NIAMS NIH HHS R01 AR054385NIGMS NIH HHS P30 GM103333
6 · The paper itself

Abstract

Both mechanical loading and intracellular autophagy play important roles in bone homeostasis; however, their relationship remains largely unexplored. The objectives of this study were to determine whether osteocytes undergo autophagy upon fluid shear stress (FSS) loading and to determine the correlation between mechanically induced autophagy and ATP metabolism. Autophagic vacuoles were observed by transmission electron microscopy (TEM) in osteocyte-like MLO-Y4 cells subjected to FSS. Increased autophagic flux was further confirmed by the increased amount of the LC3-II isoform and the degradation of p62. Fluorescent puncta distributed in the cytoplasm were observed in the GFP-LC3 transformed cells subjected to FSS. Furthermore, FSS-induced ATP release and synthesis in osteocytes were attenuated by inhibiting autophagy with 3-MA. After FSS exposure, a high ratio of cell death was observed in cultures pretreated with 3-MA, an autophagy inhibitor, with no significantly different Caspase 3/7 activity. Our results indicated that FSS induces protective autophagy in osteocytes and that mechanically induced autophagy is associated with ATP metabolism and osteocyte survival. From the clinical perspective, it may be possible to enhance skeletal cell survival with drugs that modulate the autophagic state, and the autophagy-related pathway could be a potential target for the prevention of ageing-related bone disorders.

Indexed as

AutophagyCell SurvivalAdenosine TriphosphateAnimalsCell LineMiceOsteocytesStress, MechanicalAdenosine TriphosphateATPAutophagyCell survivalFluid shear stressOsteocyte

Identifiers

PMID29096322
PMCPMC5680519
OpenAlexW2766924760

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.