ArticleJournal of lipid research2018
Quantification of 3-ketodihydrosphingosine using HPLC-ESI-MS/MS to study SPT activity in yeast
Article in Journal of lipid research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Decreased sphingolipid synthesis in children with 17q21 asthma-risk genotypes.The Journal of clinical investigation · 2020Trial
- Orm2 promotes nitrogen-induced sphingolipid production and endocytosis via Orm1 phosphorylation.Journal of lipid research · 2025Article
- Don't Be Surprised When These Surprise You: Some Infrequently Studied Sphingoid Bases, Metabolites, and Factors That Should Be Kept in Mind During Sphingolipidomic Studies.International journal of molecular sciences · 2025Review
- Lipid-Centric Approaches in Combating Infectious Diseases: Antibacterials, Antifungals and Antivirals with Lipid-Associated Mechanisms of Action.Antibiotics (Basel, Switzerland) · 2023Review
- Mass Spectrometry Analysis Reveals Lipids Induced by Oxidative Stress inMicroorganisms · 2023Article
- Convergent evolution of bacterial ceramide synthesis.Nature chemical biology · 2022Article
- Mass Spectrometric Analysis of Bioactive Sphingolipids in Fungi.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Dynamics of sphingolipids and the serine palmitoyltransferase complex in rat oligodendrocytes during myelination.Journal of lipid research · 2020Article
- Seipin negatively regulates sphingolipid production at the ER-LD contact site.The Journal of cell biology · 2019Article
- Approaches for probing and evaluating mammalian sphingolipid metabolism.Analytical biochemistry · 2019Review
- Use of isotopically labeled substrates reveals kinetic differences between human and bacterial serine palmitoyltransferase.Journal of lipid research · 2019Article
- Monitoring the SphingolipidFrontiers in cell and developmental biology · 2019Article
- Tsc3 regulates SPT amino acid choice inJournal of lipid research · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Serine palmitoyltransferase (SPT) catalyzes the rate-limiting step of condensation of L-serine and palmitoyl-CoA to form 3-ketodihydrosphingosine (3KDS). Here, we report a HPLC-ESI-MS/MS method to directly quantify 3KDS generated by SPT. With this technique, we were able to detect 3KDS at a level comparable to that of dihydrosphingosine in yeast
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.