ArticlePloS one2017
Insight into cordycepin biosynthesis of Cordyceps militaris: Comparison between a liquid surface culture and a submerged culture through transcriptomic analysis.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Biological Evaluation and Potential Applications of Secondary Metabolites from Fungi Belonging to the Cordycipitaceae Family with a Focus onJournal of fungi (Basel, Switzerland) · 2025Review
- Influence of Culture Conditions on Bioactive Compounds inFoods (Basel, Switzerland) · 2025Review
- Soy Sauce Fermentation withFoods (Basel, Switzerland) · 2025Article
- A Review of Genomic, Transcriptomic, and Proteomic Applications in Edible Fungi Biology: Current Status and Future Directions.Journal of fungi (Basel, Switzerland) · 2025Review
- A novel complementary pathway of cordycepin biosynthesis in Cordyceps militaris.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Improved Cordycepin Production by Cordyceps Militaris Using Corn Steep Liquor Hydrolysate as an Alternative Protein Nitrogen Source.Foods (Basel, Switzerland) · 2024Article
- Light-Exposed Metabolic Responses ofBiology · 2024Article
- Exploiting the roles of nitrogen sources for HEA increment inFrontiers in microbiology · 2024Article
- Effect of Air Drying on the Metabolic Profile of Fresh Wild and ArtificialFoods (Basel, Switzerland) · 2023Article
- Comparison of chemical profiles, antioxidation, inhibition of skin extracellular matrix degradation, and anti-tyrosinase activity between mycelium and fruiting body ofPharmaceutical biology · 2022Article
- Comprehensive Transcriptomic Analysis ofMycobiology · 2022Review
- Biomass and Cordycepin Production by the Medicinal MushroomJournal of fungi (Basel, Switzerland) · 2021Review
- Effects of Cordycepin inMicroorganisms · 2021Article
- Improving Hypoxia Adaption Causes Distinct Effects on Growth and Bioactive Compounds Synthesis in an Entomopathogenic FungusFrontiers in microbiology · 2021Article
- Enhanced production of cordycepin inBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Metabolic comparison of aerial and submerged mycelia formed in the liquid surface culture of Cordyceps militaris.MicrobiologyOpen · 2019Article
- Therapeutic Potential and Biological Applications of Cordycepin and Metabolic Mechanisms in Cordycepin-Producing Fungi.Molecules (Basel, Switzerland) · 2019Review
- Study of the whole genome, methylome and transcriptome of Cordyceps militaris.Scientific reports · 2019Article
- Identification of cordycepin biosynthesis-related genes through de novo transcriptome assembly and analysis inRoyal Society open science · 2018Article
- Uncovering global metabolic response to cordycepin production in Cordyceps militaris through transcriptome and genome-scale network-driven analysis.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cordyceps militaris produces cordycepin, which is known to be a bioactive compound. Currently, cordycepin hyperproduction of C. militaris was carried out in a liquid surface culture because of its low productivity in a submerged culture, however the reason was not known. In this study, 4.92 g/L of cordycepin was produced at the 15th day of C. militaris NBRC 103752 liquid surface culture, but only 1 mg/L was produced in the submerged culture. RNA-Seq was used to clarify the gene expression profiles of the cordycepin biosynthetic pathways of the submerged culture and the liquid surface culture. From this analysis, 1036 genes were shown to be upregulated and 557 genes were downregulated in the liquid surface culture compared with the submerged culture. Specifically, adenylosuccinate synthetase and phosphoribosylaminoimidazole-succinocarboxamide (SAICAR) synthase in purine nucleotide metabolism were significantly upregulated in the liquid surface culture. Thick mycelia formation in the liquid surface culture was found to induce the expression of hypoxia-related genes (GABA shunt, glutamate synthetase precursor, and succinate-semialdehyde dehydrogenase). Cytochrome P450 oxidoreductases containing heme were also found to be significantly enriched, suggesting that a hypoxic condition might be created in the liquid surface culture. These results suggest that hypoxic conditions are more suitable for cordycepin production in the liquid surface culture compared with the submerged culture. Our analysis paves the way for unraveling the cordycepin biosynthesis pathway and for improving cordycepin production in C. militaris.
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