Evidence map›Paper›PMID 29091775›Full record

ArticleCell reports2017

Single-Cell RNA-Seq Analysis of Infiltrating Neoplastic Cells at the Migrating Front of Human Glioblastoma.

Spyros Darmanis, Steven A Sloan, Derek Croote, Marco Mignardi, Sophia Chernikova, Peyman Samghababi, Ye Zhang, Norma Neff, Mark Kowarsky, Christine Caneda and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 679 papers.

0numbers the graph read from it
0cells of the map it votes in
679citing papers in PubMed
38.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

679 citing papers in PubMed, 1,046 citations in OpenAlex.

  1. Article
  2. Article
  3. Systems biology-driven drug repurposing in glioblastomaResearch in pharmaceutical sciences · 2026
    Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article

619 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 2 institutions in 1 country.

Spyros DarmanisDepartments of Bioengineering and Applied Physics, Stanford University and Chan Zuckerberg Biohub, 318 Campus Drive, Stanford, CA 94305, USA.
Steven A SloanDepartment of Neurobiology, Stanford University, 291 Campus Drive, Stanford, CA 94305, USA.
Derek CrooteDepartments of Bioengineering and Applied Physics, Stanford University and Chan Zuckerberg Biohub, 318 Campus Drive, Stanford, CA 94305, USA.
Marco MignardiDepartments of Bioengineering and Applied Physics, Stanford University and Chan Zuckerberg Biohub, 318 Campus Drive, Stanford, CA 94305, USA.
Sophia ChernikovaDepartment of Neurosurgery, Stanford University, 300 Pasteur Drive, Stanford, CA 94304, USA.
Peyman SamghababiDepartment of Pathology, Stanford University, 300 Pasteur Drive, Stanford, CA 94304, USA.
Ye ZhangDepartment of Neurobiology, Stanford University, 291 Campus Drive, Stanford, CA 94305, USA.
Norma NeffDepartments of Bioengineering and Applied Physics, Stanford University and Chan Zuckerberg Biohub, 318 Campus Drive, Stanford, CA 94305, USA.
Mark KowarskyDepartments of Bioengineering and Applied Physics, Stanford University and Chan Zuckerberg Biohub, 318 Campus Drive, Stanford, CA 94305, USA.
Christine CanedaDepartment of Neurobiology, Stanford University, 291 Campus Drive, Stanford, CA 94305, USA.
Gordon LiDepartment of Neurosurgery, Stanford University, 300 Pasteur Drive, Stanford, CA 94304, USA.
Steven D ChangDepartment of Neurosurgery, Stanford University, 300 Pasteur Drive, Stanford, CA 94304, USA.
Ian David ConnollyDepartment of Neurosurgery, Stanford University, 300 Pasteur Drive, Stanford, CA 94304, USA.
Yingmei LiDepartment of Neurosurgery, Stanford University, 300 Pasteur Drive, Stanford, CA 94304, USA.
Ben A BarresDepartment of Neurobiology, Stanford University, 291 Campus Drive, Stanford, CA 94305, USA.
Melanie Hayden GephartDepartment of Neurosurgery, Stanford University, 300 Pasteur Drive, Stanford, CA 94304, USA.
Stephen R QuakeDepartments of Bioengineering and Applied Physics, Stanford University and Chan Zuckerberg Biohub, 318 Campus Drive, Stanford, CA 94305, USA. Electronic address: quake@stanford.edu.
Stanford University · USChan Zuckerberg Initiative (United States) · US

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM007365 · NIGMS · STANFORD UNIVERSITY · PI CHUA, KATRIN F · 1985 to 2021
$33.8M
Phenotyping Astrocytes in Human Neurodevelopmental DisordersR01MH099555 · NIMH · STANFORD UNIVERSITY · PI BARRES, BEN A · 2013 to 2017
$1.9M
An Astrocytic Basis for HumanityR01NS081703 · NINDS · STANFORD UNIVERSITY · PI BARRES, BEN A · 2012 to 2016
$1.9M
(PQ1) Identifying and targeting human glioblastoma migrating in the peritumoral nicheR01CA216054 · NCI · STANFORD UNIVERSITY · PI HAYDEN GEPHART, MELANIE · 2017 to 2021
$1.7M
Identification of transcription factors that regulate astrocyte differentiationR00NS089780 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHANG, YE · 2017 to 2019
$745k
Cell-free tumor DNA in cerebral spinal fluid decodes tumors of the central nervous systemR21CA193046 · NCI · STANFORD UNIVERSITY · PI HAYDEN GEPHART, MELANIE, QUAKE, STEPHEN R · 2015 to 2017
$666k
Do Astrocytes Cause Neurodevelopmental Disorders?F30MH106261 · NIMH · STANFORD UNIVERSITY · PI SLOAN, STEVEN A · 2014 to 2016
$94k
NCI NIH HHS R01 CA216054NCI NIH HHS R21 CA193046NIGMS NIH HHS T32 GM007365NIMH NIH HHS F30 MH106261NIMH NIH HHS R01 MH099555NINDS NIH HHS R00 NS089780NINDS NIH HHS R01 NS081703
6 · The paper itself

Abstract

Glioblastoma (GBM) is the most common primary brain cancer in adults and is notoriously difficult to treat because of its diffuse nature. We performed single-cell RNA sequencing (RNA-seq) on 3,589 cells in a cohort of four patients. We obtained cells from the tumor core as well as surrounding peripheral tissue. Our analysis revealed cellular variation in the tumor's genome and transcriptome. We were also able to identify infiltrating neoplastic cells in regions peripheral to the core lesions. Despite the existence of significant heterogeneity among neoplastic cells, we found that infiltrating GBM cells share a consistent gene signature between patients, suggesting a common mechanism of infiltration. Additionally, in investigating the immunological response to the tumors, we found transcriptionally distinct myeloid cell populations residing in the tumor core and the surrounding peritumoral space. Our data provide a detailed dissection of GBM cell types, revealing an abundance of information about tumor formation and migration.

Indexed as

Brain NeoplasmsCluster AnalysisDNA Copy Number VariationsGlioblastomaHumansMagnetic Resonance ImagingRNA, NeoplasmSequence Analysis, RNASingle-Cell AnalysisRNA, NeoplasmbraincheckpointdiffuseGBMglioblastomagliomaheterogeneityinfiltratingRNA-seqsingle cell

Identifiers

PMID29091775
PMCPMC5810554
OpenAlexW2738946651

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.