Evidence map›Paper›PMID 29091749›Full record

ArticleRadiology2018

The Protein Corona around Nanoparticles Facilitates Stem Cell Labeling for Clinical MR Imaging.

Hossein Nejadnik, Seyed-Meghdad Taghavi-Garmestani, Steven J Madsen, Kai Li, Saeid Zanganeh, Phillip Yang, Morteza Mahmoudi, Heike E Daldrup-Link

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Radiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Trial
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. In vivo imaging of nanoparticle-labeled CAR T cells.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  10. Re-engineered imaging agent using biomimetic approaches.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2022
    Review
  11. Article
  12. Review
  13. A health concern regarding the protein corona, aggregation and disaggregation.Biochimica et biophysica acta. General subjects · 2019
    Review
  14. Tracking Cell Transplants in Femoral Osteonecrosis with Magnetic Resonance Imaging: A Proof-of-Concept Study in Patients.Clinical cancer research : an official journal of the American Association for Cancer Research · 2018
    Article
  15. Review
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hossein NejadnikFrom the Department of Radiology and Molecular Imaging Program at Stanford (H.N., S.M.T., K.L., S.Z., H.E.D.) and Division of Cardiovascular Medicine (P.Y., M.M.), Stanford School of Medicine, 725 Welch Rd, Room 1665, Stanford, CA 94305-5654; and Department of Materials Science and Engineering, Stanford University, Stanford, Calif (S.J.M.).
Seyed-Meghdad Taghavi-GarmestaniFrom the Department of Radiology and Molecular Imaging Program at Stanford (H.N., S.M.T., K.L., S.Z., H.E.D.) and Division of Cardiovascular Medicine (P.Y., M.M.), Stanford School of Medicine, 725 Welch Rd, Room 1665, Stanford, CA 94305-5654; and Department of Materials Science and Engineering, Stanford University, Stanford, Calif (S.J.M.).
Steven J MadsenFrom the Department of Radiology and Molecular Imaging Program at Stanford (H.N., S.M.T., K.L., S.Z., H.E.D.) and Division of Cardiovascular Medicine (P.Y., M.M.), Stanford School of Medicine, 725 Welch Rd, Room 1665, Stanford, CA 94305-5654; and Department of Materials Science and Engineering, Stanford University, Stanford, Calif (S.J.M.).
Kai LiFrom the Department of Radiology and Molecular Imaging Program at Stanford (H.N., S.M.T., K.L., S.Z., H.E.D.) and Division of Cardiovascular Medicine (P.Y., M.M.), Stanford School of Medicine, 725 Welch Rd, Room 1665, Stanford, CA 94305-5654; and Department of Materials Science and Engineering, Stanford University, Stanford, Calif (S.J.M.).
Saeid ZanganehFrom the Department of Radiology and Molecular Imaging Program at Stanford (H.N., S.M.T., K.L., S.Z., H.E.D.) and Division of Cardiovascular Medicine (P.Y., M.M.), Stanford School of Medicine, 725 Welch Rd, Room 1665, Stanford, CA 94305-5654; and Department of Materials Science and Engineering, Stanford University, Stanford, Calif (S.J.M.).
Phillip YangFrom the Department of Radiology and Molecular Imaging Program at Stanford (H.N., S.M.T., K.L., S.Z., H.E.D.) and Division of Cardiovascular Medicine (P.Y., M.M.), Stanford School of Medicine, 725 Welch Rd, Room 1665, Stanford, CA 94305-5654; and Department of Materials Science and Engineering, Stanford University, Stanford, Calif (S.J.M.).
Morteza MahmoudiFrom the Department of Radiology and Molecular Imaging Program at Stanford (H.N., S.M.T., K.L., S.Z., H.E.D.) and Division of Cardiovascular Medicine (P.Y., M.M.), Stanford School of Medicine, 725 Welch Rd, Room 1665, Stanford, CA 94305-5654; and Department of Materials Science and Engineering, Stanford University, Stanford, Calif (S.J.M.).
Heike E Daldrup-LinkFrom the Department of Radiology and Molecular Imaging Program at Stanford (H.N., S.M.T., K.L., S.Z., H.E.D.) and Division of Cardiovascular Medicine (P.Y., M.M.), Stanford School of Medicine, 725 Welch Rd, Room 1665, Stanford, CA 94305-5654; and Department of Materials Science and Engineering, Stanford University, Stanford, Calif (S.J.M.).

Funding

Nano Characterization and Nano Fabrication CoreU54CA199075 · NCI · STANFORD UNIVERSITY · PI GAMBHIR, SANJIV S · 2015 to 2019
$9.6M
Monitoring of Stem Cell Engraftment in Arthritic Joints with MR ImagingR01AR054458 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI DALDRUP-LINK, HEIKE ELIZABETH · 2008 to 2021
$6.7M
Tracking Mesenchymal Stem Cells with MR Imaging: Clinical TranslationR21AR066302 · NIAMS · STANFORD UNIVERSITY · PI DALDRUP-LINK, HEIKE ELIZABETH · 2015 to 2016
$404k
NCI NIH HHS U54 CA199075NIAMS NIH HHS R01 AR054458NIAMS NIH HHS R21 AR066302
6 · The paper itself

Abstract

Purpose To evaluate if the formation of a protein corona around ferumoxytol nanoparticles can facilitate stem cell labeling for in vivo tracking with magnetic resonance (MR) imaging. Materials and Methods Ferumoxytol was incubated in media containing human serum (group 1), fetal bovine serum (group 2), StemPro medium (group 3), protamine (group 4), and protamine plus heparin (group 5). Formation of a protein corona was characterized by means of dynamic light scattering, ζ potential, and liquid chromatography-mass spectrometry. Iron uptake was evaluated with 3,3'-diaminobenzidine-Prussian blue staining, lysosomal staining, and inductively coupled plasma spectrometry. To evaluate the effect of a protein corona on stem cell labeling, human mesenchymal stem cells (hMSCs) were labeled with the above formulations, implanted into pig knee specimens, and investigated with T2-weighted fast spin-echo and multiecho spin-echo sequences on a 3.0-T MR imaging unit. Data in different groups were compared by using a Kruskal-Wallis test. Results Compared with bare nanoparticles, all experimental groups showed significantly increased negative ζ values (from -37 to less than -10; P = .008). Nanoparticles in groups 1-3 showed an increased size because of the formation of a protein corona. hMSCs labeled with group 1-5 media showed significantly shortened T2 relaxation times compared with unlabeled control cells (P = .0012). hMSCs labeled with group 3 and 5 media had the highest iron uptake after cells labeled with group 1 medium. After implantation into pig knees, hMSCs labeled with group 1 medium showed significantly shorter T2 relaxation times than hMSCs labeled with group 2-5 media (P = .0022). Conclusion The protein corona around ferumoxytol nanoparticles can facilitate stem cell labeling for clinical cell tracking with MR imaging.

Indexed as

Magnetite NanoparticlesAnimalsCell TrackingChromatography, LiquidCulture MediaFerrosoferric OxideHumansMagnetic Resonance ImagingMesenchymal Stem CellsMesenchymal Stem Cell TransplantationParticle SizeProtein CoronaScattering, RadiationSurface PropertiesSus scrofaCulture MediaFerrosoferric OxideMagnetite NanoparticlesProtein Corona

Identifiers

PMID29091749
PMCPMC5831268

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.