ArticlePloS one2017
De novo transcriptome sequencing and assembly from apomictic and sexual Eragrostis curvula genotypes.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- A pangenome-based approach reveals genes associated with polyploidy and apomixis in Eragrostis curvula.The plant genome · 2026Article
- A Precise Reproductive Calendar of Sexual and Apomictic Genotypes ofPlants (Basel, Switzerland) · 2026Article
- Gene Discovery in Reproduction: RNA-seq Analysis of Isolated Pistils from Apomictic and Sexual Eragrostis curvula.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Gametophytic Apomixis: An Introduction.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Integrative gene expression and heterologous functional analysis identify candidate regulators of apomixis inFrontiers in plant science · 2026Article
- A sexual/apomictic consensus linkage map of Eragrostis curvula at tetraploid level.BMC plant biology · 2025Article
- New insights intoFrontiers in plant science · 2025Article
- Apomixis and the paradox of sex in plants.Annals of botany · 2024Review
- Differentially methylated genes involved in reproduction and ploidy levels in recent diploidized and tetraploidized Eragrostis curvula genotypes.Plant reproduction · 2024Article
- Apomixis: oh, what a tangled web we have!Planta · 2023Review
- From tetraploid to diploid, a pangenomic approach to identify genes lost during synthetic diploidization ofFrontiers in plant science · 2023Article
- A combined transcriptome - miRNAome approach revealed that aFrontiers in plant science · 2022Article
- Review
- Differential Methylation Patterns in Apomictic vs. Sexual Genotypes of the Diplosporous GrassPlants (Basel, Switzerland) · 2021Article
- A study of the heterochronic sense/antisense RNA representation in florets of sexual and apomictic Paspalum notatum.BMC genomics · 2021Article
- Genetic Transformation of Apomictic Grasses: Progress and Constraints.Frontiers in plant science · 2021Review
- Article
- Article
- Review
- Characterization and discovery of miRNA and miRNA targets from apomictic and sexual genotypes of Eragrostis curvula.BMC genomics · 2019Article
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8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A long-standing goal in plant breeding has been the ability to confer apomixis to agriculturally relevant species, which would require a deeper comprehension of the molecular basis of apomictic regulatory mechanisms. Eragrostis curvula (Schrad.) Nees is a perennial grass that includes both sexual and apomictic cytotypes. The availability of a reference transcriptome for this species would constitute a very important tool toward the identification of genes controlling key steps of the apomictic pathway. Here, we used Roche/454 sequencing technologies to generate reads from inflorescences of E. curvula apomictic and sexual genotypes that were de novo assembled into a reference transcriptome. Near 90% of the 49568 assembled isotigs showed sequence similarity to sequences deposited in the public databases. A gene ontology analysis categorized 27448 isotigs into at least one of the three main GO categories. We identified 11475 SSRs, and several of them were assayed in E curvula germoplasm using SSR-based primers, providing a valuable set of molecular markers that could allow direct allele selection. The differential contribution to each library of the spliced forms of several transcripts revealed the existence of several isotigs produced via alternative splicing of single genes. The reference transcriptome presented and validated in this work will be useful for the identification of a wide range of gene(s) related to agronomic traits of E. curvula, including those controlling key steps of the apomictic pathway in this species, allowing the extrapolation of the findings to other plant species.
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