ArticleEinstein (Sao Paulo, Brazil)
Low-level laser irradiation promotes proliferation of cryopreserved adipose-derived stem cells.
Article in Einstein (Sao Paulo, Brazil). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 13 citations in OpenAlex.
- Adipose-Derived Stem Cells Pretreated With Phototherapy Promote HUVECs Migration and Angiogenesis by Mediating EYA1 Activation.Physiological research · 2025Article
- NIR irradiation of human buccal fat pad adipose stem cells and its effect on TRP ion channels.Lasers in medical science · 2022Article
- Near-infrared 940-nm diode laser photobiomodulation of inflamed periodontal ligament stem cells.Lasers in medical science · 2022Article
- Effect of low power lasers on prokaryotic and eukaryotic cells under different stress condition: a review of the literature.Lasers in medical science · 2021Review
- Low-level laser and adipose-derived stem cells altered remodelling genes expression and improved collagen reorganization during tendon repair.Cell proliferation · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo evaluate the effect of low-level laser irradiation on proliferation and viability of murine adipose-derived stem cells previously submitted to cryopreservation.
methodsAdipose-derived stem cells were isolated from inguinal fat pads of three mice, submitted to cryopreservation in fetal bovine serum with 10% dimethylsulfoxide for 30 days and then thawed and maintained in normal culture conditions. Culture cells were either irradiated or not (control) with an InGaAIP diode laser at zero and 48 hours, using two different energy densities (0.5 and 1.0J/cm2). Cell proliferation was evaluated by trypan blue exclusion method and MTT assay at intervals of zero, 24, 48, and 72 hours after the first laser application. Cell viability and apoptosis of previously cryopreserved cells submitted to laser therapy were evaluated by flow cytometry.
resultsThe Irradiated Groups (0.5 and 1.0J/cm2) showed an increased cell proliferation (p<0.05) when compared to the Control Group, however no significant difference between the two energy densities was observed. Flow cytometry revealed a percentage of viable cells higher than 99% in all groups.
conclusionLow-level laser irradiation has stimulatory effects on the proliferation of adipose-derived stem cells previously submitted to cryopreservation.
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Registered trials
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