ArticleFrontiers in plant science2017
Development and Evaluation of a Barley 50k iSelect SNP Array.
Article in Frontiers in plant science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 171 papers, 1 of them a synthesis that pooled it.
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171 citing papers in PubMed, 1 synthesis or guideline pooled it, 329 citations in OpenAlex.
- New loci and candidate genes in spring two-rowed barley detected through meta-analysis of a field trial European network.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Pooled it
- Genome-wide association analysis of resistance to scald in an adapted multiparent winter malting barley population.The plant genome · 2026Article
- The role of methylation and structural variants in shaping the recombination landscape of barley.The Plant journal : for cell and molecular biology · 2026Article
- The potential of considering photosynthesis parameters in crop yield breeding by genomic prediction.Journal of experimental botany · 2026Article
- Haplotype Blocks Are Associated With Rapid Local Adaptation to Environmental Shifts in Wild Barley.Molecular ecology · 2026Article
- SNPoptimizer: a scalable genetic-algorithm framework to derive minimal discriminatory SNP panels from large genotyping datasets.Molecular breeding : new strategies in plant improvement · 2026Article
- Genome-Wide Characterization of Genetic Diversity and Population Structure in a Kazakhstani Two-Row Spring Barley Breeding Panel.International journal of molecular sciences · 2026Article
- Barley HvBODYGUARD1 controls cuticular specialisations regulated by SHINE transcription factors.The New phytologist · 2026Article
- Golden Promise-rapid, a fast-cycling and transformable barley genotype.Journal of experimental botany · 2026Article
- The RAD51 paralogue HvXRCC2 affects meiosis and recombination in barley.Journal of experimental botany · 2026Article
- Decoding the Genetic Basis of Salinity Tolerance at Germination and Seedling Traits in HEB-25 Barley NAM Population.Plants (Basel, Switzerland) · 2026Article
- Identification and genomic location of resistance to bacterial leaf streak of barley.The plant genome · 2026Article
- Vernalization regulatory network identifies potential novel functions for genes in the HvVRN2 locus.The New phytologist · 2026Article
- The SD1 locus affects primary seed dormancy in winter malting barley.The plant genome · 2026Article
- Article
- Identification and molecular mapping of a new Hessian fly resistance gene in the spring barley variety 'Golf'.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Molecular Labelling Tool for Cereal Genetic Resources Management Derived from Barley and Tetraploid Wheat Genebank-Genomics Projects.Plants (Basel, Switzerland) · 2026Article
- Unveiling the Potential of Functional Components in Hull-Less Barley Grains: Health Benefits, Structural Composition, and Genetic Advancements.Foods (Basel, Switzerland) · 2026Review
- Introgression of wild barley alleles improves seedlings salinity tolerance in the nested association mapping HEB-400 population.The plant genome · 2026Article
- Loss of E3 ligase HvST1 function substantially increases distal crossover frequency.The New phytologist · 2026Article
111 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput genotyping arrays continue to be an attractive, cost-effective alternative to sequencing based approaches. We have developed a new 50k Illumina Infinium iSelect genotyping array for barley, a cereal crop species of major international importance. The majority of SNPs on the array have been extracted from variants called in exome capture data of a wide range of European barley germplasm. We used the recently published barley pseudomolecule assembly to map the exome capture data, which allowed us to generate markers with accurate physical positions and detailed gene annotation. Markers from an existing and widely used barley 9k Infinium iSelect array were carried over onto the 50k chip for backward compatibility. The array design featured 49,267 SNP markers that converted into 44,040 working assays, of which 43,461 were scorable in GenomeStudio. Of the working assays, 6,251 are from the 9k iSelect platform. We validated the SNPs by comparing the genotype calls from the new array to legacy datasets. Rates of agreement averaged 98.1 and 93.9% respectively for the legacy 9k iSelect SNP set (Comadran et al., 2012) and the exome capture SNPs. To test the utility of the 50k chip for genetic mapping, we genotyped a segregating population derived from a Golden Promise × Morex cross (Liu et al., 2014) and mapped over 14,000 SNPs to genetic positions which showed a near exact correspondence to their known physical positions. Manual adjustment of the cluster files used by the interpreting software for genotype scoring improved results substantially, but migration of cluster files between sites led to a deterioration of results, suggesting that local adjustment of cluster files is required on a site-per-site basis. Information relating to the markers on the chip is available online at https://ics.hutton.ac.uk/50k.
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