ArticleScientific reports2017
The non-invasive exfoliated transcriptome (exfoliome) reflects the tissue-level transcriptome in a mouse model of NSAID enteropathy.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 26 citations in OpenAlex.
- Gut OncoMicrobiome Signatures (GOMS) as next-generation biomarkers for cancer immunotherapy.Nature reviews. Clinical oncology · 2023Pooled it
- Intestinal microbiota signatures of clinical response and immune-related adverse events in melanoma patients treated with anti-PD-1.Nature medicine · 2022Pooled it
- Fecal metatranscriptomics and glycomics suggest that bovine milk oligosaccharides are fully utilized by healthy adults.The Journal of nutritional biochemistry · 2020Trial
- Colonic mucosal and exfoliome transcriptomic profiling and fecal microbiome response to a flaxseed lignan extract intervention in humans.The American journal of clinical nutrition · 2019Trial
- Article
- Regulation of Paneth cell-specific genes in COVID-19 patients and SARS-CoV-2-infected mice by quantification of mRNA from exfoliated cells in stool samples.Scientific reports · 2024Article
- Integrated analysis of gut metabolome, microbiome, and exfoliome data in an equine model of intestinal injury.Microbiome · 2024Article
- Defining a timeline of colon pathologies after keratin 8 loss: rapid crypt elongation and diarrhea are followed by epithelial erosion and cell exfoliation.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- The cellular states and fates of shed intestinal cells.Nature metabolism · 2023Article
- Exfoliated epithelial cell transcriptome reflects both small and large intestinal cell signatures in piglets.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Comparative Metagenomic Analysis of Chicken Gut Microbial Community, Function, and Resistome to Evaluate Noninvasive and Cecal Sampling Resources.Animals : an open access journal from MDPI · 2021Article
- Assessing the Multivariate Relationship between the Human Infant Intestinal Exfoliated Cell Transcriptome (Exfoliome) and Microbiome in Response to Diet.Microorganisms · 2020Article
- Gut health, stress, and immunity in neonatal dairy calves: the host side of host-pathogen interactions.Journal of animal science and biotechnology · 2020Review
- Non-steroidal anti-inflammatory drugs (NSAIDs) and organ damage: A current perspective.Biochemical pharmacology · 2020Review
- Epigenomics and gene regulation in mammalian social systems.Current zoology · 2020Article
- Article
- NSAIDs disrupt intestinal homeostasis by suppressing macroautophagy in intestinal epithelial cells.Scientific reports · 2019Article
- Plant-based foods and the microbiome in the preservation of health and prevention of disease.The American journal of clinical nutrition · 2019Article
- Mapping gastrointestinal gene expression patterns in wild primates and humans via fecal RNA-seq.BMC genomics · 2019Article
- Detection and interpretation of fecal host mRNA in rural Malawian infants aged 6-12 months at risk for environmental enteric dysfunction.Experimental biology and medicine (Maywood, N.J.) · 2018Observational
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most frequently used classes of medications in the world, yet they induce an enteropathy that is associated with high morbidity and mortality. A major limitation to better understanding the pathophysiology and diagnosis of this enteropathy is the difficulty of obtaining information about the primary site of injury, namely the distal small intestine. We investigated the utility of using mRNA from exfoliated cells in stool as a means to surveil the distal small intestine in a murine model of NSAID enteropathy. Specifically, we performed RNA-Seq on exfoliated cells found in feces and compared these data to RNA-Seq from both the small intestinal mucosa and colonic mucosa of healthy control mice or those exhibiting NSAID-induced enteropathy. Global gene expression analysis, data intersection, pathway analysis, and computational approaches including linear discriminant analysis (LDA) and sparse canonical correlation analysis (CCA) were used to assess the inter-relatedness of tissue (invasive) and stool (noninvasive) datasets. These analyses revealed that the exfoliated cell transcriptome closely mirrored the transcriptome of the small intestinal mucosa. Thus, the exfoliome may serve as a non-invasive means of detecting and monitoring NSAID enteropathy (and possibly other gastrointestinal mucosal inflammatory diseases).
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Registered trials
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