ArticleScientific reports2017
On-demand optogenetic activation of human stem-cell-derived neurons.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Optogenetic control of odontoblastic differentiation in dental pulp stem cells (DPSC).Journal of dental sciences · 2026Article
- Reproducible Human Neural Circuits Printed with Single-Cell Precision Reveal the Functional Roles of Ephaptic Coupling.ACS nano · 2025Article
- XPRESSO: Rapid genetic engineering of human pluripotent stem cells for durable overexpression using a modular anti-silencing vector.Stem cell reports · 2025Article
- Genetics-Based Targeting Strategies for Precise Neuromodulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Application of optogenetic glial cells to neuron-glial communication.Frontiers in cellular neuroscience · 2023Review
- Tracking connectivity maps in human stem cell-derived neuronal networks by holographic optogenetics.Life science alliance · 2022Article
- Integrated Micro-Devices for a Lab-in-Organoid Technology Platform: Current Status and Future Perspectives.Frontiers in neuroscience · 2022Article
- Optogenetic Control of Human Stem Cell-Derived Neurons.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Long-term morphological and functional dynamics of human stem cell-derived neuronal networks on high-density micro-electrode arrays.Frontiers in neuroscience · 2022Article
- Novel Approaches Used to Examine and Control Neurogenesis in Parkinson's Disease.International journal of molecular sciences · 2021Review
- Cas9 RNP transfection by vapor nanobubble photoporation forMolecular therapy. Nucleic acids · 2021Article
- Review
- Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology.Micromachines · 2021Review
- Human stem cell-based models for studying autism spectrum disorder-related neuronal dysfunction.Molecular autism · 2020Review
- Next-generation stem cells - ushering in a new era of cell-based therapies.Nature reviews. Drug discovery · 2020Review
- Experimental and Computational Methods for the Study of Cerebral Organoids: A Review.Frontiers in neuroscience · 2019Review
- Stem cell models of human synapse development and degeneration.Molecular biology of the cell · 2018Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The widespread application of human stem-cell-derived neurons for functional studies is impeded by complicated differentiation protocols, immaturity, and deficient optogene expression as stem cells frequently lose transgene expression over time. Here we report a simple but precise Cre-loxP-based strategy for generating conditional, and thereby stable, optogenetic human stem-cell lines. These cells can be easily and efficiently differentiated into functional neurons, and optogene expression can be triggered by administering Cre protein to the cultures. This conditional expression system may be applied to stem-cell-derived neurons whenever timed transgene expression could help to overcome silencing at the stem-cell level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.