ArticleScientific reports2017
High-throughput assessment of mechanical properties of stem cell derived red blood cells, toward cellular downstream processing.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Nuclear mechanobiology rules immune cells' functions: from differentiation to cell trafficking and pathogen killing.Nucleus (Austin, Tex.) · 2026Review
- At the Pointy End of Mechanobiology: AFM for Transient Biomechanical Analysis.Advanced healthcare materials · 2025Review
- Biomechanics of circulating cellular and subcellular bioparticles: beyond separation.Cell communication and signaling : CCS · 2024Review
- Microparticles with tunable, cell-like properties for quantitative acoustic mechanophenotyping.Microsystems & nanoengineering · 2023Article
- Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates.Scientific reports · 2022Article
- Deep learning assisted mechanotyping of individual cells through repeated deformations and relaxations in undulating channels.Biomicrofluidics · 2022Article
- Article
- Pluripotency of embryonic stem cells lacking clathrin-mediated endocytosis cannot be rescued by restoring cellular stiffness.The Journal of biological chemistry · 2020Article
- Limitation of spiral microchannels for particle separation in heterogeneous mixtures: Impact of particles' size and deformability.Biomicrofluidics · 2020Article
- Effect of elastic modulus on inertial displacement of cell-like particles in microchannels.Biomicrofluidics · 2020Article
- Purifying stem cell-derived red blood cells: a high-throughput label-free downstream processing strategy based on microfluidic spiral inertial separation and membrane filtration.Biotechnology and bioengineering · 2020Article
- Optical Tweezers in Studies of Red Blood Cells.Cells · 2020Article
- Cardiomyocyte mechanodynamics under conditions of actin remodelling.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Statistics for real-time deformability cytometry: Clustering, dimensionality reduction, and significance testing.Biomicrofluidics · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Stem cell products, including manufactured red blood cells, require efficient sorting and purification methods to remove components potentially harmful for clinical application. However, standard approaches for cellular downstream processing rely on the use of specific and expensive labels (e.g. FACS or MACS). Techniques relying on inherent mechanical and physical properties of cells offer high-throughput scalable alternatives but knowledge of the mechanical phenotype is required. Here, we characterized for the first time deformability and size changes in CD34+ cells, and expelled nuclei, during their differentiation process into red blood cells at days 11, 14, 18 and 21, using Real-Time Deformability Cytometry (RT-DC) and Atomic Force Microscopy (AFM). We found significant differences (p < 0.0001; standardised mixed model) between the deformability of nucleated and enucleated cells, while they remain within the same size range. Expelled nuclei are smaller thus could be removed by size-based separation. An average Young's elastic modulus was measured for nucleated cells, enucleated cells and nuclei (day 14) of 1.04 ± 0.47 kPa, 0.53 ± 0.12 kPa and 7.06 ± 4.07 kPa respectively. Our identification and quantification of significant differences (p < 0.0001; ANOVA) in CD34+ cells mechanical properties throughout the differentiation process could enable development of new routes for purification of manufactured red blood cells.
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