ArticleNature communications2017
Molecular rheotaxis directs DNA migration and concentration against a pressure-driven flow.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Capillary-based, multifunctional manipulation of particles and fluidsJournal of physics D: Applied physics · 2024Article
- Directed colloidal assembly and banding via DC electrokinetics.Biomicrofluidics · 2023Article
- Unidirectional drying of a suspension of diffusiophoretic colloids under gravity.RSC advances · 2023Article
- Diffusiophoresis and Diffusio-osmosis into a Dead-End Channel: Role of the Concentration-Dependence of Zeta Potential.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- Enhanced Accumulation of Colloidal Particles in Microgrooved Channels via Diffusiophoresis and Steady-State Electrolyte Flows.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Microscale Diffusiophoresis of Proteins.The journal of physical chemistry. B · 2022Article
- Toward the Development of Rapid, Specific, and Sensitive Microfluidic Sensors: A Comprehensive Device Blueprint.JACS Au · 2021Review
- Acoustofluidic multi-well plates for enrichment of micro/nano particles and cells.Lab on a chip · 2020Article
- Electrode-Free Concentration and Recovery of DNA at Physiologically Relevant Ionic Concentrations.Analytical chemistry · 2020Article
- μLAS technology for DNA isolation coupled to Cas9-assisted targeting for sequencing and assembly of a 30 kb region in plant genome.Nucleic acids research · 2019Article
- Transverse migration and microfluidic concentration of DNA using Newtonian buffers.Biomicrofluidics · 2019Article
- Spontaneous Selective Preconcentration Leveraged by Ion Exchange and Imbibition through Nanoporous Medium.Scientific reports · 2019Article
- Versatile Analysis of DNA-Biomolecule Interactions in Solution by Hydrodynamic Separation and Single Molecule Detection.Analytical chemistry · 2019Article
- µLAS: Sizing of expanded trinucleotide repeats with femtomolar sensitivity in less than 5 minutes.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
In-line preconcentration techniques are used to improve the sensitivity of microfluidic DNA analysis platforms. The most common methods are electrokinetic and require an externally applied electric field. Here we describe a microfluidic DNA preconcentration technique that does not require an external field. Instead, pressure-driven flow from a fluid-filled microcapillary into a lower ionic strength DNA sample reservoir induces spontaneous DNA migration against the direction of flow. This migratory phenomenon that we call Molecular Rheotaxis initiates in seconds and results in a concentrated DNA bolus at the capillary orifice. We demonstrate the ease with which this concentration method can be integrated into a microfluidic total analysis system composed of in-line DNA preconcentration, size separation, and single-molecule detection. Paired experimental and numerical simulation results are used to delineate the parameters required to induce Molecular Rheotaxis, elucidate the underlying mechanism, and optimize conditions to achieve DNA concentration factors exceeding 10,000 fold.
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