ArticleProceedings of the National Academy of Sciences of the United States of America2017
Transcriptomes of major renal collecting duct cell types in mouse identified by single-cell RNA-seq.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 185 papers.
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Who cites it
185 citing papers in PubMed, 263 citations in OpenAlex.
- Novel Roles of GPCRs in the Renal Collecting Duct.Physiology (Bethesda, Md.) · 2026Review
- Tonicity drives collecting duct maturation in the mammalian kidney.Nature communications · 2026Article
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- Impaired Renal Base Excretion in Secretin Receptor Knock-Out Mice During Prolonged Base-Loading.Acta physiologica (Oxford, England) · 2026Article
- Dissecting hypertonicity- and NFAT5-dependent gene expression programs in mpkCCD cells.Physiological reports · 2026Article
- Longitudinal multiomic and spatial transcriptomic profiling of lupus nephritis progression in a murine model.Journal of immunology (Baltimore, Md. : 1950) · 2026Article
- Age-Related Adaptations in Renal Tubular Function in Female Rats.Acta physiologica (Oxford, England) · 2026Article
- Hmx2 and Dmrt2 Coordinate the Differentiation of Intercalated Cell Subtypes in Kidney.Journal of the American Society of Nephrology : JASN · 2026Article
- COPG1 Is a Selectively Essential Regulator of Cancer Progression and Chemoresistance via Redox Modulation and AKT Signaling.International journal of molecular sciences · 2026Article
- Review
- CREB-Family Transcription Factors and Vasopressin-Mediated Regulation of Aqp2 Gene Transcription.Journal of the American Society of Nephrology : JASN · 2026Article
- The scaffold protein IQGAP1 regulates the epithelial NaThe Journal of biological chemistry · 2025Article
- InducibleAmerican journal of physiology. Renal physiology · 2025Article
- Immunomodulatory Functions of Intercalated Cells in Kidney Autoimmunity.bioRxiv : the preprint server for biology · 2025Article
- Kcnn4/KCa3.1 inhibition blunts polycystic kidney disease progression in mouse models.JCI insight · 2025Article
- Single-cell RNA-sequencing of circulating tumour cells: A practical guide to workflow and translational applications.Cancer metastasis reviews · 2025Review
- Developmental Hypoxia Enhances Kidney Organoid Complexity and Maturity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Utilizing Omic Data to Understand Integrative Physiology.Physiology (Bethesda, Md.) · 2025Review
- Article
125 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 8 institutions in 2 countries.
Funding
Abstract
Prior RNA sequencing (RNA-seq) studies have identified complete transcriptomes for most renal epithelial cell types. The exceptions are the cell types that make up the renal collecting duct, namely intercalated cells (ICs) and principal cells (PCs), which account for only a small fraction of the kidney mass, but play critical physiological roles in the regulation of blood pressure, extracellular fluid volume, and extracellular fluid composition. To enrich these cell types, we used FACS that employed well-established lectin cell surface markers for PCs and type B ICs, as well as a newly identified cell surface marker for type A ICs, c-Kit. Single-cell RNA-seq using the IC- and PC-enriched populations as input enabled identification of complete transcriptomes of A-ICs, B-ICs, and PCs. The data were used to create a freely accessible online gene-expression database for collecting duct cells. This database allowed identification of genes that are selectively expressed in each cell type, including cell-surface receptors, transcription factors, transporters, and secreted proteins. The analysis also identified a small fraction of hybrid cells expressing aquaporin-2 and anion exchanger 1 or pendrin transcripts. In many cases, mRNAs for receptors and their ligands were identified in different cells (e.g.,
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.