ArticleAmerican journal of physiology. Endocrinology and metabolism2018
mTORC1/rpS6 regulates blood-testis barrier dynamics and spermatogenetic function in the testis in vivo.
Article in American journal of physiology. Endocrinology and metabolism, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Diurnal testosterone oscillations gate drug delivery via phase separation of ZO-1 at the blood-testis barrier in mice.Communications biology · 2026Article
- Testicular immunity.Molecular aspects of medicine · 2024Review
- Blood-testis barrier: a review on regulators in maintaining cell junction integrity between Sertoli cells.Cell and tissue research · 2024Review
- Exploring the pharmacological mechanism of Shengjing capsule on male infertility by a network pharmacology approach.BMC complementary medicine and therapies · 2022Article
- Defects of microtubule cytoskeletal organization in NOA human testes.Reproductive biology and endocrinology : RB&E · 2022Article
- Retinoic acid can improve autophagy through depression of the PI3K-Akt-mTOR signaling pathway via RARα to restore spermatogenesis in cryptorchid infertile rats.Genes & diseases · 2022Article
- Abscopal effects of thoracic X-ray radiation on spermatogenesis in mice.Frontiers in physiology · 2022Article
- Muscarinic acetylcholine receptor M5 is involved in spermatogenesis through the modification of cell-cell junctions.Reproduction (Cambridge, England) · 2021Article
- Microtubule-associated proteins (MAPs) in microtubule cytoskeletal dynamics and spermatogenesis.Histology and histopathology · 2021Review
- Rimklb mutation causes male infertility in mice.Scientific reports · 2021Article
- UropathogenicFrontiers in immunology · 2021Article
- The Non-hormonal Male Contraceptive Adjudin Exerts its Effects via MAPs and Signaling Proteins mTORC1/rpS6 and FAK-Y407.Endocrinology · 2021Article
- NC1-Peptide From Collagen α3 (IV) Chains in the Basement Membrane of Testes Regulates Spermatogenesis via p-FAK-Y407.Endocrinology · 2020Article
- Bioactive fragments of laminin and collagen chains: lesson from the testis.Reproduction (Cambridge, England) · 2020Review
- Autophagy Induced by ROS Aggravates Testis Oxidative Damage in Diabetes via Breaking the Feedforward Loop Linking p62 and Nrf2.Oxidative medicine and cellular longevity · 2020Review
- Cdc42 is involved in NC1 peptide-regulated BTB dynamics through actin and microtubule cytoskeletal reorganization.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- mTORC1/rpS6 and spermatogenic function in the testis-insights from the adjudin model.Reproductive toxicology (Elmsford, N.Y.) · 2019Article
- Mechanistic target of rapamycin (mTOR) implicated in plasticity of the reproductive axis during social status transitions.General and comparative endocrinology · 2019Article
- F5-Peptide and mTORC1/rpS6 Effectively Enhance BTB Transport Function in the Testis-Lesson From the Adjudin Model.Endocrinology · 2019Article
- mTORC1/rpS6 signaling complex modifies BTB transport function: an in vivo study using the adjudin model.American journal of physiology. Endocrinology and metabolism · 2019Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
The blood-testis barrier (BTB), conferred by Sertoli cells in the mammalian testis, is an important ultrastructure that supports spermatogenesis. Studies using animal models have shown that a disruption of the BTB leads to meiotic arrest, causing defects in spermatogenesis and male infertility. To better understand the regulation of BTB dynamics, we report findings herein to understand the role of ribosomal protein S6 (rpS6), a downstream signaling protein of mammalian target of rapamycin complex 1 (mTORC1), in promoting BTB disruption in the testis in vivo, making the barrier "leaky." Overexpression of wild-type rpS6 (rpS6-WT, the full-length cDNA cloned into the mammalian expression vector pCI-neo) and a constitutively active quadruple phosphomimetic mutant cloned into pCI-neo (p-rpS6-MT) vs. control (empty pCI-neo vector) was achieved by transfecting adult rat testes with the corresponding plasmid DNA using a Polyplus in vivo-jetPEI transfection reagent. On the basis of an in vivo functional BTB integrity assay, p-rpS6-MT was found to induce BTB disruption better than rpS6-WT did (and no effects in empty vector control), leading to defects in spermatogenesis, including loss of spermatid polarity and failure in the transport of cells (e.g., spermatids) and organelles (e.g., phagosomes), to be followed by germ exfoliation. More important, rpS6-WT and p-rpS6-MT exert their disruptive effects through changes in the organization of actin- and microtubule (MT)-based cytoskeletons, which are mediated by changes in the spatiotemporal expression of actin- and MT-based binding and regulatory proteins. In short, mTORC1/rpS6 signaling complex is a regulator of spermatogenesis and BTB by modulating the organization of the actin- and MT-based cytoskeletons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.