ArticleNucleic acids research2017
DNA-assisted oligomerization of pore-forming toxin monomers into precisely-controlled protein channels.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Large-Diameter DNA-Scaffolded Nanopores Enabled by Loosely Packed Peptides for Single-Molecule Sensing.Angewandte Chemie (International ed. in English) · 2026Article
- Membrane-Spanning Nanopores Formed from Nucleic Acids.Chemical reviews · 2026Review
- Deconvoluting the Effect of Cell-Penetrating Peptides for Enhanced and Controlled Insertion of Large-Scale DNA Nanopores.ACS applied materials & interfaces · 2024Article
- Lipid vesicle-based molecular robots.Lab on a chip · 2024Review
- Recent Advances in DNA Origami-Engineered Nanomaterials and Applications.Chemical reviews · 2023Review
- Functionalized DNA-Origami-Protein Nanopores Generate Large Transmembrane Channels with Programmable Size-Selectivity.Journal of the American Chemical Society · 2023Article
- A Light-Triggered Synthetic Nanopore for Controlling Molecular Transport Across Biological Membranes.Angewandte Chemie (International ed. in English) · 2022Article
- Applications of DNA-Functionalized Proteins.International journal of molecular sciences · 2021Review
- A Primer on the oxDNA Model of DNA: When to Use it, How to Simulate it and How to Interpret the Results.Frontiers in molecular biosciences · 2021Review
- Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics.APL bioengineering · 2020Review
- Engineering Lipid Membranes with Programmable DNA Nanostructures.Advanced biosystems · 2020Review
- Design and synthesis of pleated DNA origami nanotubes with adjustable diameters.Nucleic acids research · 2019Article
- Synthetic protein-conductive membrane nanopores built with DNA.Nature communications · 2019Article
- Article
- Pharmacological Targeting of Pore-Forming Toxins as Adjunctive Therapy for Invasive Bacterial Infection.Toxins · 2018Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a novel approach for creating membrane-spanning protein-based pores. The construction principle is based on using well-defined, circular DNA nanostructures to arrange a precise number of pore-forming protein toxin monomers. We can thereby obtain, for the first time, protein pores with specifically set diameters. We demonstrate this principle by constructing artificial alpha-hemolysin (αHL) pores. The DNA/αHL hybrid nanopores composed of twelve, twenty or twenty-six monomers show stable insertions into lipid bilayers during electrical recordings, along with steady, pore size-dependent current levels. Our approach successfully advances the applicability of nanopores, in particular towards label-free studies of single molecules in large nanoscaled biological structures.
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