ArticlePloS one2017
Comparison of the transcriptomic analysis between two Chinese white pear (Pyrus bretschneideri Rehd.) genotypes of different stone cells contents.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 50 citations in OpenAlex.
- Gap-free genome assemblies of two Pyrus bretschneideri cultivars and GWAS analyses identify a CCCH zinc finger protein as a key regulator of stone cell formation in pear fruit.Plant communications · 2025Article
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- Comparative analysis of POD genes and their expression under multiple hormones in Pyrus bretschenedri.BMC genomic data · 2024Article
- Multi-omics analysis provides insights into the mechanism underlying fruit color formation inFrontiers in plant science · 2024Article
- Review
- Comparative Genomic Analysis ofInternational journal of molecular sciences · 2022Article
- Analysis of the β-Glucosidase Family Reveals Genes Involved in the Lignification of Stone Cells in Chinese White Pear (Frontiers in plant science · 2022Article
- Comparative genomic analysis of thePeerJ · 2022Article
- Morphological and Developmental Features of Stone Cells inFrontiers in plant science · 2022Article
- Production of reactive oxygen species by PuRBOHF is critical for stone cell development in pear fruit.Horticulture research · 2021Article
- Comparative Genomic Analysis ofFrontiers in genetics · 2021Article
- Comparative genomic analysis of superoxide dismutase (Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Biochemical characterization and expression analysis of lignification in two pear (Pyrus ussuriensis Maxim.) varieties with contrasting stone cell content.Protoplasma · 2020Article
- Transcriptomic analysis of early fruit development in Chinese white pear (Pyrus bretschneideri Rehd.) and functional identification of PbCCR1 in lignin biosynthesis.BMC plant biology · 2019Article
- Article
- Family-1 UDP glycosyltransferases in pear (Pyrus bretschneideri): Molecular identification, phylogenomic characterization and expression profiling during stone cell formation.Molecular biology reports · 2019Article
- Article
- Article
- Article
- Transcriptome Analysis Provides Insights into Gingerol Biosynthesis in Ginger (The plant genome · 2018Article
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10 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Stone cell content is thought to be one of the key determinants for fruit quality in pears. However, the molecular mechanism of stone cell development remains poorly understood. In this study, we found that the stone cell clusters (SCCs) distribution and area in 'Dangshan Su' (with abundant stone cells) were higher as compared to 'Lianglizaosu' (low stone cell content bud sport of 'Dangshan Su') based on the histochemical staining, and the correlations of lignin content with stone cell content and SCC area was significant. The fruits of 'Dangshan Su' and 'Lianglizaosu' at three different developmental stages (23 and 55 days after flowering and mature) were sampled for comparative transcriptome analysis to explore the metabolic pathways associated with stone cell development. A total of 42444 unigenes were obtained from two varieties, among which 7203 differentially expressed genes (DEGs) were identified by comparison of the six transcriptomes. Specifically, many DEGs associated with lignin biosynthesis were identified, including coumaroylquinate 3-monooxygenase (C3H), shikimate O-hydroxycinnamoyltransferase (HCT), ferulate 5-hydroxylase (F5H), cinnamyl alcohol dehydrogenase (CAD) and peroxidase (POD), as well as genes related to carbon metabolism, such as sorbitol dehydrogenase-like (SDH-like) and ATP-dependent 6-phosphofructokinase (ATP-PFK). At the peak of the stone cell content (55 days after flowering), the expression level of these genes in 'Dangshan Su' was significantly increased compared with 'Lianglizaosu', indicating that these genes were closely related to stone cell development. We validated the transcriptional levels of 33 DEGs using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The results were consistent with the transcriptome analysis, indicating the reliability of transcriptome data. In addition, subcellular localization analysis of three DEGs in lignin synthesis (PbC3H, PbF5H and PbPOD) revealed that these proteins are mainly distributed in the cell membrane and cytoplasm. These results provide new insights into the molecular mechanism of stone cell formation.
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