ArticleBiochemistry2017
Comparison of Five Protein Engineering Strategies for Stabilizing an α/β-Hydrolase.
Article in Biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Article
- Decoding Protein Stabilization: Impact on Aggregation, Solubility, and Unfolding Mechanisms.Journal of chemical information and modeling · 2025Article
- KnowVolution of an Efficient Polyamidase through Molecular Dynamics Simulations of Incrementally Docked Oligomeric Substrates.ChemSusChem · 2025Article
- GArNet: A Genetic Algorithm-Based Protein Redesign Approach to Optimize Mutation Combinations Informed by Network Theory.Journal of chemical information and modeling · 2025Article
- Engineering Protein Dynamics through Mutational Energy Landscape Traps.Journal of chemical information and modeling · 2025Article
- Design of ancestral mammalian alkaline phosphatase bearing high stability and productivity.Applied and environmental microbiology · 2024Article
- Enzymatic Degradation of Deoxynivalenol with the Engineered Detoxification Enzyme Fhb7.JACS Au · 2024Article
- Computational Enzyme Redesign Enhances Tolerance to Denaturants for Peptide C-Terminal Amidation.JACS Au · 2024Article
- Protein Stability: Enhancement and Measurement.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Enzymatic Conjugation of Modified RNA Fragments by Ancestral RNA Ligase AncT4_2.Applied and environmental microbiology · 2022Article
- Leveraging intrinsic flexibility to engineer enhanced enzyme catalytic activity.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Engineering and evaluation of thermostableEngineering in life sciences · 2022Article
- Review
- Improving the Thermostability and Activity of Transaminase FromFrontiers in chemistry · 2021Article
- Decoding the intricate network of molecular interactions of a hyperstable engineered biocatalyst.Chemical science · 2020Article
- Improving the catalytic thermostability of3 Biotech · 2020Article
- Larger active site in an ancestral hydroxynitrile lyase increases catalytically promiscuous esterase activity.PloS one · 2020Article
- Consensus Design of an Evolved High-Redox Potential Laccase.Frontiers in bioengineering and biotechnology · 2020Article
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4 authors.
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Abstract
A review of the previous stabilization of α/β-hydrolase fold enzymes revealed many different strategies, but no comparison of strategies on the same enzyme. For this reason, we compared five strategies to identify stabilizing mutations in a model α/β-hydrolase fold enzyme, salicylic acid binding protein 2, to reversible denaturation by urea and to irreversible denaturation by heat. The five strategies included one location agnostic approach (random mutagenesis using error-prone polymerase chain reaction), two structure-based approaches [computational design (Rosetta, FoldX) and mutation of flexible regions], and two sequence-based approaches (addition of proline at locations where a more stable homologue has proline and mutation to consensus). All strategies identified stabilizing mutations, but the best balance of success rate, degree of stabilization, and ease of implementation was mutation to consensus. A web-based automated program that predicts substitutions needed to mutate to consensus is available at http://kazlab.umn.edu .
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