ReviewFrontiers in plant science2017
Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review.
Review in Frontiers in plant science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Advanced Nanomaterial-Based Electrochemical Biosensing of Loop-Mediated Isothermal Amplification Products.Biosensors · 2025Article
- Future perspectives of GMO detection in agriculture: strategies for electrochemical nucleic acid detection.Mikrochimica acta · 2025Review
- Detection of Soybean GMO Events Using Two Multiplex Droplet Digital PCR Assays.Journal of AOAC International · 2025Article
- An Accurate, Rapid and Cost-Effective Method for T-nos Detection Based on CRISPR/Cas12a.Foods (Basel, Switzerland) · 2023Article
- A CRISPR-based lateral flow assay for plant genotyping and pathogen diagnostics.Plant biotechnology journal · 2022Article
- Collaborative Ring Trial of the Applicability of a Reference Plasmid DNA Calibrant in the Quantitative Analysis of GM Maize Event MON810.Foods (Basel, Switzerland) · 2022Article
- Genetically Modified Soybean Detection Using a Biosensor Electrode with a Self-Assembled Monolayer of Gold Nanoparticles.Biosensors · 2022Article
- Rapid detection of genetically modified products based on CRISPR-Cas12a combined with recombinase polymerase amplification.Current research in food science · 2022Article
- An Event-Specific Real-Time PCR Method for Measuring Transgenic Lysozyme Goat Content in Trace Samples.Foods (Basel, Switzerland) · 2021Article
- Using chemiluminescence imaging of cells (CLIC) for relative protein quantification.Scientific reports · 2020Article
- Development of a sensitive monoclonal antibody-based sandwich ELISA to detect Vip3Aa in genetically modified crops.Biotechnology letters · 2020Article
- Event-specific qualitative polymerase chain reaction analysis for two T-DNA copies in genetically modified orangePlant cell, tissue and organ culture · 2020Article
- Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection.BMC biotechnology · 2019Article
- Safety Assessment of Genetically Modified Feed: Is There Any Difference From Food?Frontiers in plant science · 2019Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As long as the genetically modified crops are gaining attention globally, their proper approval and commercialization need accurate and reliable diagnostic methods for the transgenic content. These diagnostic techniques are mainly divided into two major groups, i.e., identification of transgenic (1) DNA and (2) proteins from GMOs and their products. Conventional methods such as PCR (polymerase chain reaction) and enzyme-linked immunosorbent assay (ELISA) were routinely employed for DNA and protein based quantification respectively. Although, these Techniques (PCR and ELISA) are considered as significantly convenient and productive, but there is need for more advance technologies that allow for high throughput detection and the quantification of GM event as the production of more complex GMO is increasing day by day. Therefore, recent approaches like microarray, capillary gel electrophoresis, digital PCR and next generation sequencing are more promising due to their accuracy and precise detection of transgenic contents. The present article is a brief comparative study of all such detection techniques on the basis of their advent, feasibility, accuracy, and cost effectiveness. However, these emerging technologies have a lot to do with detection of a specific event, contamination of different events and determination of fusion as well as stacked gene protein are the critical issues to be addressed in future.
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Registered trials
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