ArticleScientific reports2017
New method for the identification of arbuscular mycorrhizal fungi by proteomic-based biotyping of spores using MALDI-TOF-MS.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Speciation analysis of fungi by liquid atmospheric pressure MALDI mass spectrometry.Analytical and bioanalytical chemistry · 2025Article
- Diversity of Arbuscular Mycorrhizal Fungi of the Rhizosphere ofJournal of fungi (Basel, Switzerland) · 2024Article
- Seed protein biotyping in Amaranthus species: a tool for rapid identification of weedy amaranths of concern.Plant methods · 2023Article
- Phenol and Polyaromatic Hydrocarbons Are Stronger Drivers Than Host Plant Species in Shaping the Arbuscular Mycorrhizal Fungal Component of the Mycorrhizosphere.International journal of molecular sciences · 2022Article
- Identification and Typing of Strains of Wood-Rotting Basidiomycetes by Protein Profiling Using MALDI-TOF MS.Biotech (Basel (Switzerland)) · 2022Article
- Methods for assessing the quality of AM fungal bio-fertilizer: Retrospect and future directions.World journal of microbiology & biotechnology · 2022Review
- Development of a taxon-discriminating molecular marker to trace and quantify a mycorrhizal inoculum in roots and soils of agroecosystems.Folia microbiologica · 2021Article
- Article
- Identification and dereplication of endophytic Colletotrichum strains by MALDI TOF mass spectrometry and molecular networking.Scientific reports · 2020Article
- Identification to species level of live single microalgal cells from plankton samples with matrix-free laser/desorption ionization mass spectrometry.Metabolomics : Official journal of the Metabolomic Society · 2020Article
- Rapid MALDI-TOF MS identification of commercial truffles.Scientific reports · 2019Article
- Sensitive approach and future perspectives in microscopic patterns of mycorrhizal roots.Scientific reports · 2019Article
- Is a mixture of arbuscular mycorrhizal fungi better for plant growth than single-species inoculants?Mycorrhiza · 2019Article
- Identification ofBiology methods & protocols · 2019Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arbuscular mycorrhizal fungi (AMF, Glomeromycota) are mutualistic symbionts associated with majority of land plants. These fungi play an important role in plant growth, but their taxonomic identification remains a challenge for academic research, culture collections and inoculum producers who need to certify their products. Identification of these fungi was traditionally performed based on their spore morphology. DNA sequence data have successfully been used to study the evolutionary relationships of AMF, develop molecular identification tools and assess their diversity in the environment. However, these methods require considerable expertise and are not well-adapted for "routine" quality control of culture collections and inoculum production. Here, we show that Matrix-Assisted Laser Desorption Ionisation Time of Flight Mass Spectrometry proteomic-based biotyping is a highly efficient approach for AMF identification. Nineteen isolates belonging to fourteen species, seven genera and five families were clearly differentiated by MALDI biotyping at the species level, and intraspecific differentiation was achieved for the majority. AMF identification by MALDI biotyping could be highly useful, not only for research but also in agricultural and environmental applications. Fast, accurate and inexpensive molecular mass determination and the possibility of automation make MALDI-TOF-MS a real alternative to conventional morphological and molecular methods for AMF identification.
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