ArticleParasites & vectors2017
Molecular typing of Trichomonas vaginalis isolates by actin gene sequence analysis and carriage of T. vaginalis viruses.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 30 citations in OpenAlex.
- Molecular Diagnosis ofJournal of tropical medicine · 2026Review
- Presence of Protozoan Viruses in Vaginal Samples from Pregnant Women and Their Association with Trichomoniasis.Pathogens (Basel, Switzerland) · 2025Article
- Microbial Matryoshka: Addressing the Relationship between Pathogenic Flagellated Protozoans and Their RNA Viral Endosymbionts (FamilyVeterinary sciences · 2024Review
- Characterization of Actin-based Genotypes and Mycoplasma Endosymbionts of Trichomonas vaginalis Isolated in Sapporo, Japan.Acta parasitologica · 2024Article
- Microscopic and molecular detection ofFrontiers in microbiology · 2024Article
- Prevalence and Genotype ofJournal of tropical medicine · 2023Article
- Article
- Characterisation ofInternational journal of molecular sciences · 2022Article
- Molecular typing of the actin gene ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Omics Analyses ofGenes · 2022Article
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- Identification ofReports of biochemistry & molecular biology · 2021Article
- A review onSouthern African journal of infectious diseases · 2021Review
- Genotypic Variation inInfectious diseases in obstetrics and gynecology · 2020Article
- Prevalence of double-stranded RNA virus in Trichomonas vaginalis isolated in Italy and association with the symbiont Mycoplasma hominis.Parasitology research · 2019Article
- Trichomonas vaginalis Virus Among Women With Trichomoniasis and Associations With Demographics, Clinical Outcomes, and Metronidazole Resistance.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
- Prevalence and genetic diversity of Trichomonas vaginalis clinical isolates in a targeted population in Xinxiang City, Henan Province, China.Parasites & vectors · 2018Article
- Microsatellite-Based Genotyping, Analysis of Population Structure, Presence ofIranian journal of parasitologyArticle
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Authors and funding
5 authors at 3 institutions in 2 countries.
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Abstract
backgroundThe protozoan parasite Trichomonas vaginalis is the most common non-viral, sexually transmitted pathogen. Although T. vaginalis is highly prevalent among women in Kenya, there is lack of data regarding genetic diversity of isolates currently in circulation in Kenya.
methodsTyping was performed on 22 clinical isolates of T. vaginalis collected from women attending the antenatal care clinic at Kilifi County Hospital, Kenya, in 2015. Genotyping followed a previously proposed restriction fragment length polymorphism (RFLP) scheme, which involved in silico cleavage of the amplified actin gene by HindII, MseI and RsaI restriction enzymes. Phylogenetic analysis of all the sequences was performed to confirm the results obtained by RFLP-analysis and to assess the diversity within the RFLP genotypes. Additionally, we determined carriage of the four different types of Trichomonas vaginalis viruses (TVVs) by polymerase chain reaction.
resultsIn silico RFLP-analysis revealed five actin genotypes; 50.0% of the isolates were of actin genotype E, 27.3% of actin genotype N, 13.6% of actin genotype G and 4.5% of actin genotypes I and P. Phylogenetic analysis was in agreement with the RFLP-analysis, with the different actin genotypes clustering together. Prevalence of TVVs was 43.5% (95% confidence interval, CI: 23.2-65.5). TVV1 was the most prevalent, present in 39.1% of the strains and 90% of the T. vaginalis isolates which harbored TVVs had more than one type of TVV. None of the isolates of actin genotype E harbored any TVV.
conclusionThe presence of five actin genotypes in our study suggests notable diversity among T. vaginalis isolates occurring among pregnant women in Kilifi, Kenya. Isolates of the most prevalent actin genotype E lacked TVVs. We found no association between T. vaginalis genotype, carriage of TVVs and symptoms. Further studies with higher number of strains should be conducted in order to corroborate these results.
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