ArticleMicrobial cell factories2017
Production of 3-hydroxypropionic acid in engineered Methylobacterium extorquens AM1 and its reassimilation through a reductive route.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 69 citations in OpenAlex.
- Repair of DNA and protein damages caused by formaldehyde improves methanol assimilation.Fundamental research · 2026Article
- Engineering de novo formatotrophy in the non-model yeastNature synthesis · 2026Article
- Adaptively evolvedApplied and environmental microbiology · 2025Article
- A novel engineered strain of Methylorubrum extorquens for methylotrophic production of glycolic acid.Microbial cell factories · 2024Article
- Constructing efficient bacterial cell factories to enable one-carbon utilization based on quantitative biology: A review.Quantitative biology (Beijing, China) · 2024Review
- Heterologous production of 3-hydroxypropionic acid in Methylorubrum extorquens by introducing the mcr gene via a multi-round chromosomal integration system based on cre-lox71/lox66 and transposon.Microbial cell factories · 2024Article
- Expression of toxic genes in Methylorubrum extorquens with a tightly repressed, cumate-inducible promoter.Antonie van Leeuwenhoek · 2023Article
- Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid.Microbial cell factories · 2023Article
- Characterization of CSynthetic and systems biotechnology · 2023Article
- Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs.Synthetic and systems biotechnology · 2023Article
- Engineering 3-Hydroxypropionic Acid Production from Glucose inJournal of fungi (Basel, Switzerland) · 2023Article
- Improvement of dicarboxylic acid production with Methylorubrum extorquens by reduction of product reuptake.Applied microbiology and biotechnology · 2022Article
- A pBBR1-based vector with IncP group plasmid compatibility for Methylorubrum extorquens.MicrobiologyOpen · 2022Article
- Developing methylotrophic microbial platforms for a methanol-based bioindustry.Frontiers in bioengineering and biotechnology · 2022Review
- Aerobic Utilization of Methanol for Microbial Growth and Production.Advances in biochemical engineering/biotechnology · 2022Article
- Translating advances in microbial bioproduction to sustainable biotechnology.Frontiers in bioengineering and biotechnology · 2022Review
- Benchmarking recombinant Pichia pastoris for 3-hydroxypropionic acid production from glycerol.Microbial biotechnology · 2021Article
- Biosynthesis pathways and strategies for improving 3-hydroxypropionic acid production in bacteria.World journal of microbiology & biotechnology · 2021Review
- Levulinic Acid-Inducible and Tunable Gene Expression System forFrontiers in bioengineering and biotechnology · 2021Article
- Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae.Nature communications · 2020Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background3-Hydroxypropionic acid (3-HP) is an important platform chemical, serving as a precursor for a wide range of industrial applications such as the production of acrylic acid and 1,3-propanediol. Although Escherichia coli or Saccharomyces cerevisiae are the primary industrial microbes for the production of 3-HP, alternative engineered hosts have the potential to generate 3-HP from other carbon feedstocks. Methylobacterium extorquens AM1, a facultative methylotrophic α-proteobacterium, is a model system for assessing the possibility of generating 3-HP from one-carbon feedstock methanol.
resultsHere we constructed a malonyl-CoA pathway by heterologously overexpressing the mcr gene to convert methanol into 3-HP in M. extorquens AM1. The engineered strains demonstrated 3-HP production with initial titer of 6.8 mg/l in shake flask cultivation, which was further improved to 69.8 mg/l by increasing the strength of promoter and mcr gene copy number. In vivo metabolic analysis showed a significant decrease of the acetyl-CoA pool size in the strain with the highest 3-HP titer, suggesting the supply of acetyl-CoA is a potential bottleneck for further improvement. Notably, 3-HP was rapidly degraded after the transition from exponential phase to stationary phase. Metabolomics analysis showed the accumulation of intracellular 3-hydroxypropionyl-CoA at stationary phase with the addition of 3-HP into the cultured medium, indicating 3-HP was first converted to its CoA derivatives. In vitro enzymatic assay and β-alanine pathway dependent
conclusionsWe demonstrated the feasibility of constructing the malonyl-CoA pathway in M. extorquens AM1 to generate 3-HP. Furthermore, we showed that a reductive route coupled with the ethylmalonyl-CoA pathway was the major channel responsible for degradation of the 3-HP during the growth transition. Engineered M. extorquens AM1 represents a good platform for 3-HP production from methanol.
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