Evidence map›Paper›PMID 29084543›Full record

ArticleMicrobial cell factories2017

Flow cytometric quantification, sorting and sequencing of methanogenic archaea based on F

Johannes Lambrecht, Nicolas Cichocki, Thomas Hübschmann, Christin Koch, Hauke Harms, Susann Müller

Abstract read
In one paragraph

Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

  1. Detection and quantification of rumen methanogens using FApplied and environmental microbiology · 2026
    Article
  2. Review
  3. Review
  4. Article
  5. Heliyon · 2024
    Review
  6. Article
  7. Phenotypic and genomic characterization ofApplied and environmental microbiology · 2024
    Article
  8. Article
  9. Microbiology spectrum · 2024
    Article
  10. Microorganisms · 2024
    Article
  11. Article
  12. Fc-MBL-modified FeMikrochimica acta · 2022
    Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Review
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Johannes LambrechtDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research-UFZ, Permoserstr. 15, 04318, Leipzig, Germany.
Nicolas CichockiDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research-UFZ, Permoserstr. 15, 04318, Leipzig, Germany.
Thomas HübschmannDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research-UFZ, Permoserstr. 15, 04318, Leipzig, Germany.
Christin KochDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research-UFZ, Permoserstr. 15, 04318, Leipzig, Germany.
Hauke HarmsDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research-UFZ, Permoserstr. 15, 04318, Leipzig, Germany.
Susann MüllerDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research-UFZ, Permoserstr. 15, 04318, Leipzig, Germany. susann.mueller@ufz.de.ORCID http://orcid.org/0000-0003-2060-6256

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe widely established production of CH

resultsIn this study we present an autofluorescence-based, flow cytometric method for the fast low-cost quantification of methanogenic archaea in complex microbial communities and crude substrates. The method was applied to a methanogenic enrichment culture (MEC) and digester samples (DS). The methanogenic archaea were quantified using the distinct fluorescence of their cofactor F

conclusionsThe presented method allows for fast and reliable quantification of methanogenic archaea in microbial communities under authentic digester conditions and can thus be useful for process monitoring and control in biogas digesters.

Indexed as

ArchaeaArchaeal ProteinsBenzothiazolesBiofuelsBiomassDiaminesFlow CytometryMethaneMicroscopy, FluorescenceOrganic ChemicalsQuinolinesRNA, Ribosomal, 16SSequence Analysis, DNAArchaeal ProteinsBenzothiazolesBiofuelsDiaminesMethaneOrganic ChemicalsQuinolinesRNA, Ribosomal, 16SSYBR Green I16S rDNA sequencingAnaerobic digestionAutofluorescenceBiogasF420Methanogenic archaeaProcess monitoringSingle cell analytics

Identifiers

PMID29084543
PMCPMC5663091

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.