ArticleThe Journal of clinical investigation2017
Protein kinase A determines platelet life span and survival by regulating apoptosis.
Article in The Journal of clinical investigation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 65 citations in OpenAlex.
- Iloprost Concentration-Dependently Attenuates Platelet Function and Apoptosis by Elevating PKA Activity.Journal of cellular and molecular medicine · 2025Article
- Platelet-derived extracellular vesicles in cardiovascular disease and treatment - from maintaining homeostasis to targeted drug delivery.Current opinion in hematology · 2025Review
- Single-Cell Spatial Transcriptomics Unveils Platelet-Fueled Cycling Macrophages for Kidney Fibrosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Structural analysis of platelet fragments and extracellular vesicles produced by apheresis platelets during storage.Blood advances · 2024Article
- Interaction networks explain holoenzyme allostery in Protein Kinase A.Kinases and phosphatases · 2023Article
- Inhibition of GPIb-α-mediated apoptosis signaling enables cold storage of platelets.Haematologica · 2023Article
- Preanalytical conditions for multiparameter platelet flow cytometry.Research and practice in thrombosis and haemostasis · 2023Article
- Protein Phosphatase 1 γ Modulates Steady-State BAD Phosphorylation and Murine Platelet Survival.Thrombosis and haemostasis · 2023Article
- Fluorescence quenching-based immunological probe for ticagrelor monitoring.Frontiers in bioengineering and biotechnology · 2023Article
- A simplified machine learning model utilizing platelet-related genes for predicting poor prognosis in sepsis.Frontiers in immunology · 2023Article
- Review
- CPT2 K79 acetylation regulates platelet life span.Blood advances · 2022Article
- Unfolded Protein Response Differentially Modulates the Platelet Phenotype.Circulation research · 2022Article
- Analysis of preplatelets and their barbell platelet derivatives by imaging flow cytometry.Blood advances · 2022Article
- Autophagy Ameliorates Reactive Oxygen Species-Induced Platelet Storage Lesions.Oxidative medicine and cellular longevity · 2022Article
- Association of Platelet Desialylation and Circulating Follicular Helper T Cells in Patients With Thrombocytopenia.Frontiers in immunology · 2022Article
- A novel mechanism of thrombocytopenia by PS exposure through TMEM16F in sphingomyelin synthase 1 deficiency.Blood advances · 2021Article
- Platelet Innate Immune Receptors and TLRs: A Double-Edged Sword.International journal of molecular sciences · 2021Review
- Carbamazepine Induces Platelet Apoptosis and Thrombocytopenia Through Protein Kinase A.Frontiers in pharmacology · 2021Article
- Calcium Ion Chelation Preserves Platelet Function During Cold Storage.Arteriosclerosis, thrombosis, and vascular biology · 2021Article
Corrections and comments
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Authors and funding
23 authors at 2 institutions in 2 countries.
Funding
Abstract
Apoptosis delimits platelet life span in the circulation and leads to storage lesion, which severely limits the shelf life of stored platelets. Moreover, accumulating evidence indicates that platelet apoptosis provoked by various pathological stimuli results in thrombocytopenia in many common diseases. However, little is known about how platelet apoptosis is initiated or regulated. Here, we show that PKA activity is markedly reduced in platelets aged in vitro, stored platelets, and platelets from patients with immune thrombocytopenia (ITP), diabetes, and bacterial infections. Inhibition or genetic ablation of PKA provoked intrinsic programmed platelet apoptosis in vitro and rapid platelet clearance in vivo. PKA inhibition resulted in dephosphorylation of the proapoptotic protein BAD at Ser155, resulting in sequestration of prosurvival protein BCL-XL in mitochondria and subsequent apoptosis. Notably, PKA activation protected platelets from apoptosis induced by storage or pathological stimuli and elevated peripheral platelet levels in normal mice and in a murine model of ITP. Therefore, these findings identify PKA as a homeostatic regulator of platelet apoptosis that determines platelet life span and survival. Furthermore, these results suggest that regulation of PKA activity represents a promising strategy for extending platelet shelf life and has profound implications for the treatment of platelet number-related diseases and disorders.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.