ArticleJournal of medical virology2018
Metagenomic analysis of viral diversity in respiratory samples from patients with respiratory tract infections in Kuwait.
Article in Journal of medical virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 3 of them syntheses that pooled it.
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Who cites it
23 citing papers in PubMed, 3 syntheses or guidelines pooled it, 26 citations in OpenAlex.
- Microbiome and One Health in GCC countries: current status, research gaps, and future directions.Frontiers in microbiology · 2026Pooled it
- Diagnostic accuracy of metagenomic next-generation sequencing in diagnosing infectious diseases: a meta-analysis.Scientific reports · 2022Pooled it
- Detection of respiratory viruses directly from clinical samples using next-generation sequencing: A literature review of recent advances and potential for routine clinical use.Reviews in medical virology · 2022Pooled it
- Metagenomic sequencing identifies potential respiratory pathogens in PCR-negative subset of surveillance samples.Scientific reports · 2026Article
- Metagenomic next-generation sequencing for the diagnosis and evaluation of pediatric pleural effusion: a case series.BMC pulmonary medicine · 2026Article
- Human bocavirus: As an emerging respiratory pathogen.World journal of virology · 2025Review
- Acute Respiratory Infections (ARIs): Current Etiological Perspectives and Advances in Viral Metagenomics-A Review.Viruses · 2025Review
- The respiratory tract virome: unravelling the role of viral dark matter in respiratory health and disease.European respiratory review : an official journal of the European Respiratory Society · 2025Review
- Molecular Epidemiology and Clinical Characteristics of an Outbreak on Respiratory Virus Coinfection in Gansu, China.Viruses · 2024Article
- Metagenomics characterization of respiratory viral RNA pathogens in children under five years with severe acute respiratory infection in the Free State, South Africa.Journal of medical virology · 2023Article
- Antibiotic Resistance Genes in Aerosols: Baseline from Kuwait.International journal of molecular sciences · 2023Article
- Evaluation of extraction and enrichment methods for recovery of respiratory RNA viruses in a metagenomics approach.Journal of virological methods · 2023Article
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- Bacterial and fungal communities in indoor aerosols from two Kuwaiti hospitals.Frontiers in microbiology · 2022Article
- Detection of respiratory viruses in adults with suspected COVID-19 in Kuala Lumpur, Malaysia.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2021Article
- Composition of nasal bacterial community and its seasonal variation in health care workers stationed in a clinical research laboratory.PloS one · 2021Article
- Phenotypes and personalized medicine in the acute respiratory distress syndrome.Intensive care medicine · 2020Review
- Article
- Analysis of viral diversity in stool samples from infants and children with acute gastroenteritis in Kuwait using Metagenomics approach.Virology journal · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A metagenomic approach based on target independent next-generation sequencing has become a known method for the detection of both known and novel viruses in clinical samples. This study aimed to use the metagenomic sequencing approach to characterize the viral diversity in respiratory samples from patients with respiratory tract infections. We have investigated 86 respiratory samples received from various hospitals in Kuwait between 2015 and 2016 for the diagnosis of respiratory tract infections. A metagenomic approach using the next-generation sequencer to characterize viruses was used. According to the metagenomic analysis, an average of 145, 019 reads were identified, and 2% of these reads were of viral origin. Also, metagenomic analysis of the viral sequences revealed many known respiratory viruses, which were detected in 30.2% of the clinical samples. Also, sequences of non-respiratory viruses were detected in 14% of the clinical samples, while sequences of non-human viruses were detected in 55.8% of the clinical samples. The average genome coverage of the viruses was 12% with the highest genome coverage of 99.2% for respiratory syncytial virus, and the lowest was 1% for torque teno midi virus 2. Our results showed 47.7% agreement between multiplex Real-Time PCR and metagenomics sequencing in the detection of respiratory viruses in the clinical samples. Though there are some difficulties in using this method to clinical samples such as specimen quality, these observations are indicative of the promising utility of the metagenomic sequencing approach for the identification of respiratory viruses in patients with respiratory tract infections.
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Registered trials
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