Evidence map›Paper›PMID 29082099›Full record

ArticleBiomedical optics express2017

Label free monitoring of megakaryocytic development and proplatelet formation in vitro.

Dimitra Pouli, Lorenzo Tozzi, Carlo A Alonzo, Zhiyi Liu, David L Kaplan, Alessandra Balduini, Irene Georgakoudi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Biophotonics feature: introduction.Biomedical optics express · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 2 countries.

Dimitra PouliDepartment of Biomedical Engineering, Tufts University, 4 Colby St., 02155 Medford MA, USA.
Lorenzo TozziDepartment of Biomedical Engineering, Tufts University, 4 Colby St., 02155 Medford MA, USA.
Carlo A AlonzoDepartment of Biomedical Engineering, Tufts University, 4 Colby St., 02155 Medford MA, USA.
Zhiyi LiuDepartment of Biomedical Engineering, Tufts University, 4 Colby St., 02155 Medford MA, USA.
David L KaplanDepartment of Biomedical Engineering, Tufts University, 4 Colby St., 02155 Medford MA, USA.
Alessandra BalduiniDepartment of Biomedical Engineering, Tufts University, 4 Colby St., 02155 Medford MA, USA.
Irene GeorgakoudiDepartment of Biomedical Engineering, Tufts University, 4 Colby St., 02155 Medford MA, USA.
Tufts University · US

Funding

In vitro bioreactor system for platelet formationR01EB016041 · NIBIB · TUFTS UNIVERSITY MEDFORD · PI BALDUINI, ALESSANDRA, KAPLAN, DAVID L. · 2012 to 2020
$2.7M
NIBIB NIH HHS R01 EB016041
6 · The paper itself

Abstract

Megakaryopoiesis and platelet production are complex biological processes that require tight regulation of successive lineage commitment steps and are ultimately responsible for maintaining and renewing the pool of circulating platelets in the blood. Despite major advancements in the understanding of megakaryocytic biology, the detailed mechanisms driving megakaryocytic differentiation have yet to be elucidated. Here we show that automated image analysis algorithms applied to two-photon excited fluorescence (TPEF) images can non-invasively monitor structural and metabolic megakaryocyte behavior changes occurring during differentiation and platelet formation in vitro. Our results demonstrate that high-contrast, label-free two photon imaging holds great potential in studying the underlying physiological processes controlling the intricate process of platelet production.

Indexed as

(170.0170) Medical optics and biotechnology(170.1470) Blood or tissue constituent monitoring(170.3880) Medical and biological imaging(180.2520) Fluorescence microscopy(190.0190) Nonlinear optics

Identifiers

PMID29082099
PMCPMC5654814
OpenAlexW2757680155

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.