Evidence map›Paper›PMID 29081510›Full record

ReviewNature reviews. Nephrology2017

Isolation and characterization of urinary extracellular vesicles: implications for biomarker discovery.

Michael L Merchant, Ilse M Rood, Jeroen K J Deegens, Jon B Klein

Open access · greenAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Nature reviews. Nephrology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 261 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
261citing papers in PubMed, 5 pooled it
15.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

261 citing papers in PubMed, 5 syntheses or guidelines pooled it, 503 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Guideline
  5. Pooled it
  6. Trial
  7. Review
  8. Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Review
  19. Review
  20. Article

201 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 4 institutions in 2 countries.

Michael L MerchantDivision of Nephrology and Hypertension, Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA.
Ilse M RoodRadboud University Medical Center, Radboud Institute of Health Science, Department of Nephrology, Nijmegen 6500HB, The Netherlands.
Jeroen K J DeegensRadboud University Medical Center, Radboud Institute of Health Science, Department of Nephrology, Nijmegen 6500HB, The Netherlands.
Jon B KleinDivision of Nephrology and Hypertension, Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA.
Louisville VA Medical Center · USRadboud University Medical Center · NLRadboud University Nijmegen · NLUniversity of Louisville · US

Funding

Role of oxidized linoleic acid metabolites in the pathogenesis of alcoholic liver diseaseP20GM113226 · NIGMS · UNIVERSITY OF LOUISVILLE · PI GHARE, SMITA S · 2016 to 2025
$24.1M
The Role of Nutrition in the Development/Progression of Alcohol-Induced Organ InjuryP50AA024337 · NIAAA · UNIVERSITY OF LOUISVILLE · PI SHIRISH S BARVE · 2016 to 2026
$17.9M
Novel biomarker validation and dosing algorithms for anemia management in ESRDR01DK091584 · NIDDK · UNIVERSITY OF LOUISVILLE · PI BRIER, MICHAEL E, MERCHANT, MICHAEL L · 2011 to 2015
$2.6M
Integrating Proteomics and Metabolomics to Understand Pediatric Glomerular Disease Pathophysiology and PrognosisR01DK110077 · NIDDK · RESEARCH INST NATIONWIDE CHILDREN'S HOSP · PI KLEIN, JON, SMOYER, WILLIAM E · 2016 to 2020
$1.8M
NIAAA NIH HHS P50 AA024337NIDDK NIH HHS R01 DK091584NIDDK NIH HHS R01 DK110077NIGMS NIH HHS P20 GM113226
6 · The paper itself

Abstract

Urine is a valuable diagnostic medium and, with the discovery of urinary extracellular vesicles, is viewed as a dynamic bioactive fluid. Extracellular vesicles are lipid-enclosed structures that can be classified into three categories: exosomes, microvesicles (or ectosomes) and apoptotic bodies. This classification is based on the mechanisms by which membrane vesicles are formed: fusion of multivesicular bodies with the plasma membranes (exosomes), budding of vesicles directly from the plasma membrane (microvesicles) or those shed from dying cells (apoptotic bodies). During their formation, urinary extracellular vesicles incorporate various cell-specific components (proteins, lipids and nucleic acids) that can be transferred to target cells. The rigour needed for comparative studies has fueled the search for optimal approaches for their isolation, purification, and characterization. RNA, the newest extracellular vesicle component to be discovered, has received substantial attention as an extracellular vesicle therapeutic, and compelling evidence suggests that ex vivo manipulation of microRNA composition may have uses in the treatment of kidney disorders. The results of these studies are building the case that urinary extracellular vesicles act as mediators of renal pathophysiology. As the field of extracellular vesicle studies is burgeoning, this Review focuses on primary data obtained from studies of human urine rather than on data from studies of laboratory animals or cultured immortalized cells.

Indexed as

BiomarkersExtracellular VesiclesHumansKidneyLipid MetabolismProteomeTranscriptomeUrinalysisUrologic DiseasesBiomarkersProteome

Identifiers

PMID29081510
PMCPMC5941934
OpenAlexW2765280408

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.