ArticleNature communications2017
Multi-wavelength anomalous diffraction de novo phasing using a two-colour X-ray free-electron laser with wide tunability.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Viscoelastic characterization of the lipid cubic phase provides insights into high-viscosity extrusion injection for XFEL experiments.Scientific reports · 2025Article
- Heavy-element damage seeding in proteins under XFEL illumination.Journal of synchrotron radiation · 2025Article
- Exploring the potential of a bioassembler for protein crystallization in space.NPJ microgravity · 2025Article
- Generation of time-synchronized two-color X-ray free-electron laser pulses using phase shifters.Scientific reports · 2023Article
- Article
- Discerning best practices in XFEL-based biological crystallography - standards for nonstandard experiments.IUCrJ · 2021Review
- Article
- High-throughput in situ experimental phasing.Acta crystallographica. Section D, Structural biology · 2020Article
- Illumination guidelines for ultrafast pump-probe experiments by serial femtosecond crystallography.Nature methods · 2020Article
- SPIND-TC: an indexing method for two-color X-ray diffraction data.Acta crystallographica. Section A, Foundations and advances · 2020Article
- Article
- Viscosity-adjustable grease matrices for serial nanocrystallography.Scientific reports · 2020Article
- Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the human plasma glycoprotein afamin.Acta crystallographica. Section D, Structural biology · 2019Article
- SAD phasing of XFEL data depends critically on the error model.Acta crystallographica. Section D, Structural biology · 2019Article
- The complementarity of serial femtosecond crystallography and MicroED for structure determination from microcrystals.Current opinion in structural biology · 2019Review
- An outlook on using serial femtosecond crystallography in drug discovery.Expert opinion on drug discovery · 2019Review
- Protein crystal structure determination with the crystallophore, a nucleating and phasing agent.Journal of applied crystallography · 2019Article
- Article
- Crystallography on a chip - without the chip: sheet-on-sheet sandwich.Acta crystallographica. Section D, Structural biology · 2018Article
- Article
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22 authors.
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Abstract
Serial femtosecond crystallography at X-ray free-electron lasers (XFELs) offers unprecedented possibilities for macromolecular structure determination of systems prone to radiation damage. However, de novo structure determination, i.e., without prior structural knowledge, is complicated by the inherent inaccuracy of serial femtosecond crystallography data. By its very nature, serial femtosecond crystallography data collection entails shot-to-shot fluctuations in X-ray wavelength and intensity as well as variations in crystal size and quality that must be averaged out. Hence, to obtain accurate diffraction intensities for de novo phasing, large numbers of diffraction patterns are required, and, concomitantly large volumes of sample and long X-ray free-electron laser beamtimes. Here we show that serial femtosecond crystallography data collected using simultaneous two-colour X-ray free-electron laser pulses can be used for multiple wavelength anomalous dispersion phasing. The phase angle determination is significantly more accurate than for single-colour phasing. We anticipate that two-colour multiple wavelength anomalous dispersion phasing will enhance structure determination of difficult-to-phase proteins at X-ray free-electron lasers.
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