ArticleScientific reports2017
Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Vascularization of Human iPSC-Derived Kidney Organoids Using Perfusion Culture, Pre-Vascularized Collagen Scaffolds, and Decellularized Extracellular Matrix.Advanced healthcare materials · 2026Article
- Stromal vascular fraction self-assembles vascularized osteogenic organoids with immunomodulatory functions.Bioactive materials · 2026Article
- Harnessing the angiogenic potential of adipose-derived stromal vascular fraction cells with perfusion cell seeding.Stem cell research & therapy · 2025Article
- Label-Free Prediction of Fluorescently Labeled Fibrin Networks.Biomaterials research · 2025Article
- Transplantation of adipose tissue-derived microvascular fragments promotes therapy of critical limb ischemia.Biomaterials research · 2023Article
- Upscaled Skeletal Muscle Engineered Tissue with In Vivo Vascularization and Innervation Potential.Bioengineering (Basel, Switzerland) · 2023Article
- A perfusion-based three-dimensional cell culture system to model alveolar rhabdomyosarcoma pathological features.Scientific reports · 2023Article
- Growth Factor-Free Vascularization of Marine-Origin Collagen Sponges Using Cryopreserved Stromal Vascular Fractions from Human Adipose Tissue.Marine drugs · 2022Article
- Culturing patient-derived malignant hematopoietic stem cells in engineered and fully humanized 3D niches.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Long-Term Severe In Vitro Hypoxia Exposure Enhances the Vascularization Potential of Human Adipose Tissue-Derived Stromal Vascular Fraction Cell Engineered Tissues.International journal of molecular sciences · 2021Article
- 3D Collagen Hydrogel Promotes In Vitro Langerhans Islets Vascularization through ad-MVFs Angiogenic Activity.Biomedicines · 2021Article
- The Duo of Osteogenic and Angiogenic Differentiation in ADSC-Derived Spheroids.Frontiers in cell and developmental biology · 2021Article
- Wholly vascularized millimeter-sized engineered tissues by cell-sized microscaffolds.Materials today. Bio · 2020Article
- Combining Vascularization Strategies in Tissue Engineering: The Faster Road to Success?Frontiers in bioengineering and biotechnology · 2020Article
- Prevascularization of collagen-glycosaminoglycan scaffolds: stromal vascular fraction versus adipose tissue-derived microvascular fragments.Journal of biological engineering · 2018Article
- Plasticity of Adipose Tissue-Derived Stem Cells and Regulation of Angiogenesis.Frontiers in physiology · 2018Review
- Engineering of fully humanized and vascularized 3D bone marrow niches sustaining undifferentiated human cord blood hematopoietic stem and progenitor cells.Journal of tissue engineeringArticle
- Microvascular fragment spheroids: Three-dimensional vascularization units for tissue engineering and regeneration.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro recapitulation of an organotypic stromal environment, enabling efficient angiogenesis, is crucial to investigate and possibly improve vascularization in regenerative medicine. Our study aims at engineering the complexity of a vascular milieu including multiple cell-types, a stromal extracellular matrix (ECM), and molecular signals. For this purpose, the human adipose stromal vascular fraction (SVF), composed of a heterogeneous mix of pericytes, endothelial/stromal progenitor cells, was cultured under direct perfusion flow on three-dimensional (3D) collagen scaffolds. Perfusion culture of SVF-cells reproducibly promoted in vitro the early formation of a capillary-like network, embedded within an ECM backbone, and the release of numerous pro-angiogenic factors. Compared to static cultures, perfusion-based engineered constructs were more rapidly vascularized and supported a superior survival of delivered cells upon in vivo ectopic implantation. This was likely mediated by pericytes, whose number was significantly higher (4.5-fold) under perfusion and whose targeted depletion resulted in lower efficiency of vascularization, with an increased host foreign body reaction. 3D-perfusion culture of SVF-cells leads to the engineering of a specialized milieu, here defined as an angiogenic niche. This system could serve as a model to investigate multi-cellular interactions in angiogenesis, and as a module supporting increased grafted cell survival in regenerative medicine.
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