ArticleThe Science of the total environment2018
Analysis of airborne microbial communities using 16S ribosomal RNA: Potential bias due to air sampling stress.
Article in The Science of the total environment, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
9 citing papers in PubMed, 34 citations in OpenAlex.
- Comparison of Three Bioaerosol Samplers for Bacterial Diversity.Journal of aerosol science · 2026Article
- Advancing transcriptomic profiling of airborne bacteria.Applied and environmental microbiology · 2025Review
- Advances in single-cell sequencing technology in microbiome research.Genes & diseases · 2024Review
- Sampling efficiency and nucleic acid stability during long-term sampling with different bioaerosol samplers.Environmental monitoring and assessment · 2024Article
- Characterization and DNA Stable-Isotope Probing of Methanotrophic Bioaerosols.Microbiology spectrum · 2022Article
- Canopy Position Has a Stronger Effect than Tree Species Identity on Phyllosphere Bacterial Diversity in a Floodplain Hardwood Forest.Microbial ecology · 2021Article
- Performance evaluation of high-volume electret filter air samplers in aerosol microbiome research.Environmental microbiome · 2020Article
- Bioaerosol Sampling: Classical Approaches, Advances, and Perspectives.Aerosol science and technology : the journal of the American Association for Aerosol Research · 2020Article
- Bioaerosol Sampler Choice Should Consider Efficiency and Ability of Samplers To Cover Microbial Diversity.Applied and environmental microbiology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A limited number of studies have been conducted to analyze ribosomal RNA (rRNA, present in the ribosome) in bioaerosol samples to identify currently or potentially active airborne microbes, although its genomic counterpart, the rRNA gene (on the chromosome) has been frequently targeted for airborne microbial community analysis. A knowledge gap still exists regarding whether the bioaerosol rRNA abundances are affected by the bioaerosol collection process. We investigated the effect of air sampling stress on the measurement and characterization of 16S rRNA for bioaerosols in the laboratory and field experiments using quantitative polymerase chain reaction (qPCR) and high-throughput sequencing techniques. In a laboratory study, known quantities of freshly grown Escherichia coli cells were spiked onto the filter of a Button Aerosol Sampler and into liquids of BioSampler and SpinCon air samplers and then exposed to sampling stress when the samplers were operated for 2h. We found that the recovered cellular 16S rRNA abundance as determined by qPCR was dependent on sampler type. Further, two devices (Button Aerosol Sampler and BioSampler) that exhibited markedly different efficiency in preserving 16S rRNA were employed in an outdoor environment to collect bioaerosols simultaneously on eight days in two different seasons. The abundance of 16S rRNA in the outdoor air sample (1.3×10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.