ArticleJournal of structural biology2018
Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells.
Article in Journal of structural biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Islet ultrastructure: past achievements and future directions.Diabetologia · 2026Review
- Cryogenic electron tomography by the numbers: Charting underexplored lineages in structural cell biology.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Large transient assemblies of Apaf1 constitute the apoptosome in cells.Nature communications · 2025Article
- Preparation of Vitrified Mammalian Cells for In Situ Cryo-Electron Tomography.Current protocols · 2025Article
- Combinatorial selective ER-phagy remodels the ER during neurogenesis.Nature cell biology · 2024Article
- Green Synthesis of Carbon Nanoparticles (CNPs) from Biomass for Biomedical Applications.International journal of molecular sciences · 2023Review
- Reducing Frost during Cryoimaging Using a Hygroscopic Ice Frame.ACS omega · 2022Article
- Bridging length scales from molecules to the whole organism by cryoCLEM and cryoET.Faraday discussions · 2022Article
- In situ cryo-FIB/SEM Specimen Preparation Using the Waffle Method.Bio-protocol · 2022Article
- Quantitative, in situ visualization of intracellular insulin vesicles in pancreatic beta cells.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Reduction of autofluorescence in whole adult worms of Schistosoma japonicum for immunofluorescence assay.Parasites & vectors · 2021Article
- The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes.Science (New York, N.Y.) · 2021Article
- Quantifying nanodiamonds biodistribution in whole cells with correlative iono-nanoscopy.Nature communications · 2021Article
- CorRelator: Interactive software for real-time high precision cryo-correlative light and electron microscopy.Journal of structural biology · 2021Article
- In Situ Imaging and Structure Determination of Biomolecular Complexes Using Electron Cryo-Tomography.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Correlated cryogenic fluorescence microscopy and electron cryo-tomography shows that exogenous TRIM5α can form hexagonal lattices or autophagy aggregates in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- 3D histopathology of human tumours by fast clearing and ultramicroscopy.Scientific reports · 2020Article
- Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells.Science advances · 2020Article
- Bright sub-20-nm cathodoluminescent nanoprobes for electron microscopy.Nature nanotechnology · 2019Article
- Cryo-SOFI enabling low-dose super-resolution correlative light and electron cryo-microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
In cryogenic correlated light and electron microscopy (cryo-CLEM), frozen targets of interest are identified and located on EM grids by fluorescence microscopy and then imaged at higher resolution by cryo-EM. Whilst working with these methods, we discovered that a variety of mammalian cells exhibit strong punctate autofluorescence when imaged under cryogenic conditions (80 K). Autofluorescence originated from multilamellar bodies (MLBs) and secretory granules. Here we describe a method to distinguish fluorescent protein tags from these autofluorescent sources based on the narrower emission spectrum of the former. The method is first tested on mitochondria and then applied to examine the ultrastructural variability of secretory granules within insulin-secreting pancreatic beta-cell-derived INS-1E cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.