ArticleMicrobiome2017
Comparing microbiotas in the upper aerodigestive and lower respiratory tracts of lambs.
Article in Microbiome, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed.
- Comparative Lung Bacterial Community Profiles in Pneumonic and Non-Pneumonic Akkaraman Lambs.Animals : an open access journal from MDPI · 2026Article
- Microbial landscapes in dairy cow diseases: from localized dysbiosis to inter-organ axes.NPJ biofilms and microbiomes · 2026Review
- Impact of whole-herd vaccination on a caprine coxiellosis outbreak: a longitudinal study ofFrontiers in microbiology · 2026Article
- Multidimensional Phenotypic and Microbiome Studies Uncover an Association Between Reduced Feed Efficiency in Sheep During Mycoplasmal Pneumonia and Microbial Crosstalk Within the Rumen-Lung Axis.Veterinary sciences · 2025Article
- Metabarcoding analysis of the microbiota in flocks naturally infected byOne health (Amsterdam, Netherlands) · 2025Article
- Application of multi-omics technology in pathogen identification and resistance gene screening of sheep pneumonia.BMC genomics · 2025Article
- Article
- Etiology, persistence, and risk factors of subclinical mastitis in a meat-producing sheep flock.Journal of animal science · 2024Article
- Insights into microbial compositions of the respiratory tract of neonatal dairy calves in a longitudinal probiotic trial through 16S rRNA sequencing.Frontiers in microbiology · 2024Article
- What Makes the Gut-Lung Axis Working? From the Perspective of Microbiota and Traditional Chinese Medicine.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2024Review
- Characterization of Lung Microbiomes in Pneumonic Hu Sheep Using Culture Technique and 16S rRNA Gene Sequencing.Animals : an open access journal from MDPI · 2023Article
- The spatial dissimilarities and connections of the microbiota in the upper and lower respiratory tract of beef cattle.Frontiers in cellular and infection microbiology · 2023Article
- Topography of the respiratory, oral, and guttural pouch bacterial and fungal microbiotas in horses.Journal of veterinary internal medicine · 2023Article
- Probiotics in milk replacer affect the microbiome of the lung in neonatal dairy calves.Frontiers in microbiology · 2023Article
- Alterations of lower respiratory tract microbiome and short-chain fatty acids in different segments in lung cancer: a multiomics analysis.Frontiers in cellular and infection microbiology · 2023Article
- Assessing the effects of experimental bacterial challenge with Pasteurella multocida and ampicillin on the respiratory microbiota of pre-weaned Holstein calves.Veterinary microbiology · 2022Article
- Bovine respiratory microbiota of feedlot cattle and its association with disease.Veterinary research · 2022Review
- Technical note: overcoming host contamination in bovine vaginal metagenomic samples with nanopore adaptive sequencing.Journal of animal science · 2022Article
- The lung microbiome in lung transplantation.The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation · 2021Review
- Episodic Aspiration with Oral Commensals Induces a MyD88-dependent, Pulmonary T-Helper Cell Type 17 Response that Mitigates Susceptibility toAmerican journal of respiratory and critical care medicine · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
backgroundRecently, the importance of the lung microbiota during health and disease has been examined in humans and in small animal models. Whilst sheep have been proposed as an appropriate large animal model for studying the pathophysiology of a number of important human respiratory diseases, it is clearly important to continually define the limits of agreement between these systems as new concepts emerge. In humans, it has recently been established that the lung microbiota is seeded by microbes from the oral cavity. We sought to determine whether the same was true in sheep.
resultsWe took lung fluid and upper aerodigestive tract (oropharyngeal) swab samples from 40 lambs (7 weeks old). DNA extraction was performed, and the V2-V3 region of the 16S rRNA gene was amplified by PCR then sequenced via Illumina Miseq. Oropharyngeal swabs were either dominated by bacteria commonly associated with the rumen or by bacteria commonly associated with the upper aerodigestive tract. Lung microbiota samples did not resemble either the upper aerodigestive tract samples or reagent-only controls. Some rumen-associated bacteria were found in lung fluids, indicating that inhalation of ruminal bacteria does occur. We also identified several bacteria which were significantly more abundant in lung fluids than in the upper aerodigestive tract swabs, the most predominant of which was classified as Staphylococcus equorum.
conclusionsIn contrast to humans, we found that the lung microbiota of lambs is dissimilar to that of the upper aerodigestive tract, and we suggest that this may be related to physiological and anatomical differences between sheep and humans. Understanding the comparative physiology and anatomy underlying differences in lung microbiota between species will provide a foundation upon which to interpret changes associated with disease and/or environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.