ArticleProceedings of the National Academy of Sciences of the United States of America2017
Requirement of the fusogenic micropeptide myomixer for muscle formation in zebrafish.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 70 citations in OpenAlex.
- Characterization and cellularity of myofibers during early development of Magalobrama amblycephala.Fish physiology and biochemistry · 2026Article
- Changes in gene expression at the early stages of barramundi (Lates calcarifer) muscle cell line development.Molecular biology reports · 2026Article
- Myomaker and Myomixer are required for craniofacial myoblast fusion in zebrafish.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Article
- Cell Surface Thiol Engineering Mechanoregulates Myogenic Differentiation via the FAK-PI3K-AKT Axis.Advanced healthcare materials · 2026Article
- Dynamic expression and differential requirement of the myocyte fusogen Myomixer during distinct myogenic episodes in the zebrafish.Biology open · 2025Article
- Abnormal mitochondrial structure and function in brown adipose tissue of SLC35A4-MP knockout mice.Science advances · 2025Article
- Bi-allelic MYMX variants cause a syndromic congenital myopathy with recognizable facial palsy, growth restriction, and dysmorphism.European journal of human genetics : EJHG · 2025Article
- The miR-6240 target gene Igf2bp3 promotes myoblast fusion by enhancing myomaker mRNA stability.Cellular & molecular biology letters · 2024Article
- Ectopic expression of Myomaker and Myomixer in slow muscle cells induces slow muscle fusion and myofiber death.Journal of genetics and genomics = Yi chuan xue bao · 2024Article
- Spatiotemporal coordination of actin regulators generates invasive protrusions in cell-cell fusion.Nature cell biology · 2024Article
- Review
- Molecular regulation of myocyte fusion.Current topics in developmental biology · 2024Review
- Small Open Reading Frame-Encoded Micro-Peptides: An Emerging Protein World.International journal of molecular sciences · 2023Review
- Multi-faceted CRISPR/Cas technological innovation aspects in the framework of 3P medicine.The EPMA journal · 2023Review
- Myomaker and Myomixer Characterization in Gilthead Sea Bream under Different Myogenesis Conditions.International journal of molecular sciences · 2022Article
- Loss of Myomixer Results in Defective Myoblast Fusion, Impaired Muscle Growth, and Severe Myopathy in Zebrafish.Marine biotechnology (New York, N.Y.) · 2022Article
- Phosphatidylserine orchestrates Myomerger membrane insertions to drive myoblast fusion.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Evolution of a chordate-specific mechanism for myoblast fusion.Science advances · 2022Article
- The cellular architecture and molecular determinants of the zebrafish fusogenic synapse.Developmental cell · 2022Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Skeletal muscle formation requires fusion of mononucleated myoblasts to form multinucleated myofibers. The muscle-specific membrane proteins myomaker and myomixer cooperate to drive mammalian myoblast fusion. Whereas myomaker is highly conserved across diverse vertebrate species, myomixer is a micropeptide that shows relatively weak cross-species conservation. To explore the functional conservation of myomixer, we investigated the expression and function of the zebrafish myomixer ortholog. Here we show that myomixer expression during zebrafish embryogenesis coincides with myoblast fusion, and genetic deletion of myomixer using CRISPR/Cas9 mutagenesis abolishes myoblast fusion in vivo. We also identify myomixer orthologs in other species of fish and reptiles, which can cooperate with myomaker and substitute for the fusogenic activity of mammalian myomixer. Sequence comparison of these diverse myomixer orthologs reveals key amino acid residues and a minimal fusogenic peptide motif that is necessary for promoting cell-cell fusion with myomaker. Our findings highlight the evolutionary conservation of the myomaker-myomixer partnership and provide insights into the molecular basis of myoblast fusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.