ArticleThe Plant journal : for cell and molecular biology2018
Affinity proteomics reveals extensive phosphorylation of the Brassica chromosome axis protein ASY1 and a network of associated proteins at prophase I of meiosis.
Article in The Plant journal : for cell and molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 52 citations in OpenAlex.
- Loss of E3 ligase HvST1 function substantially increases distal crossover frequency.The New phytologist · 2026Article
- SCEP3 initiates synapsis and implements crossover interference in Arabidopsis.Nature plants · 2025Article
- Seeding the meiotic DNA break machinery and initiating recombination on chromosome axes.Nature communications · 2024Article
- TurboID-based proteomic profiling of meiotic chromosome axes in Arabidopsis thaliana.Nature plants · 2023Article
- ThePNAS nexus · 2023Article
- Identification, characterization, and rescue of CRISPR/Cas9 generated wheat SPO11-1 mutants.Plant biotechnology journal · 2023Article
- ASYNAPSIS 1 ensures crossover fidelity in polyploid wheat by promoting homologous recombination and suppressing non-homologous recombination.Frontiers in plant science · 2023Article
- Differentiated function and localisation of SPO11-1 and PRD3 on the chromosome axis during meiotic DSB formation in Arabidopsis thaliana.PLoS genetics · 2022Article
- FANCM promotes class I interfering crossovers and suppresses class II non-interfering crossovers in wheat meiosis.Nature communications · 2022Article
- Interfered chromosome pairing at high temperature promotes meiotic instability in autotetraploid Arabidopsis.Plant physiology · 2022Article
- Telomerase Interaction Partners-Insight from Plants.International journal of molecular sciences · 2021Article
- ATM controls meiotic DNA double-strand break formation and recombination and affects synaptonemal complex organization in plants.The Plant cell · 2021Article
- ZYP1 is required for obligate cross-over formation and cross-over interference inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Distal Bias of Meiotic Crossovers in Hexaploid Bread Wheat Reflects Spatio-Temporal Asymmetry of the Meiotic Program.Frontiers in plant science · 2021Article
- Network Topological Analysis for the Identification of Novel Hubs in Plant Nutrition.Frontiers in plant science · 2021Article
- Ubiquitination in Plant Meiosis: Recent Advances and High Throughput Methods.Frontiers in plant science · 2021Review
- Rewiring Meiosis for Crop Improvement.Frontiers in plant science · 2021Review
- The Formation of Bivalents and the Control of Plant Meiotic Recombination.Frontiers in plant science · 2021Review
- Article
- A novel allele of ASY3 is associated with greater meiotic stability in autotetraploid Arabidopsis lyrata.PLoS genetics · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During meiosis, the formation of crossovers (COs) generates genetic variation and provides physical links that are essential for accurate chromosome segregation. COs occur in the context of a proteinaceous chromosome axis. The transcriptomes and proteomes of anthers and meiocytes comprise several thousand genes and proteins, but because of the level of complexity relatively few have been functionally characterized. Our understanding of the physical and functional interactions between meiotic proteins is also limited. Here we use affinity proteomics to analyse the proteins that are associated with the meiotic chromosome axis protein, ASY1, in Brassica oleracea anthers and meiocytes. We show that during prophase I ASY1 and its interacting partner, ASY3, are extensively phosphorylated, and we precisely assign phosphorylation sites. We identify 589 proteins that co-immunoprecipitate with ASY1. These correspond to 492 Arabidopsis orthologues, over 90% of which form a coherent protein-protein interaction (PPI) network containing known and candidate meiotic proteins, including proteins more usually associated with other cellular processes such as DNA replication and proteolysis. Mutant analysis confirms that affinity proteomics is a viable strategy for revealing previously unknown meiotic proteins, and we show how the PPI network can be used to prioritise candidates for analysis. Finally, we identify another axis-associated protein with a role in meiotic recombination. Data are available via ProteomeXchange with identifier PXD006042.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.