ArticlePloS one2017
RNAi-derived transgenic resistance to Mungbean yellow mosaic India virus in cowpea.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- RNA interference mediated disease resistance in vegetable crops: fundamental discovery to current trends.Molecular biology reports · 2026Review
- Geminiviral-CR-gRNA expressed in cowpea efficiently edited MYMV and MYMIV genome to provide resistance against cowpea yellow mosaic disease without hampering plant growth and yield.BMC plant biology · 2026Article
- Hairpin-RNA spray confers resistance to mungbean yellow mosaic India virus in mungbean.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- A review of factors affecting the success of geminivirus infectious clones.Plant cell reports · 2025Review
- RNA silencing: the future potential strategy for engineering virus resistance in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Review
- Geminivirus diseases of legumes in India: current status and approaches for management.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Unveiling the dynamic relationship of viruses and/or symbiotic bacteria with plant resilience in abiotic stress.Stress biology · 2024Review
- Major viral diseases in grain legumes: designing disease resistant legumes from plant breeding and OMICS integration.Frontiers in plant science · 2023Review
- RNA Interference: Promising Approach to Combat Plant Viruses.International journal of molecular sciences · 2022Review
- Strategies for Efficient RNAi-Based Gene Silencing of Viral Genes for Disease Resistance in Plants.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A Method for Developing RNAi-Derived Resistance in Cowpea Against Geminiviruses.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Influence of virus-host interactions on plant response to abiotic stress.Plant cell reports · 2021Review
- RNA interference and crop protection against biotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Genetic transformation of legumes: an update.Plant cell reports · 2021Review
- Optimized RNA-Silencing Strategies forPlants (Basel, Switzerland) · 2021Article
- Construction of Infectious Clones of Begomoviruses: Strategies, Techniques and Applications.Biology · 2021Review
- Current Status and Potential of RNA Interference for the Management of Tomato Spotted Wilt Virus and Thrips Vectors.Pathogens (Basel, Switzerland) · 2021Review
- Induction of resistance to sugarcane mosaic virus by RNA interference targeting coat protein gene silencing in transgenic sugarcane.Molecular biology reports · 2021Article
- RNAi technology for management of banana bunchy top disease.Food and energy security · 2020Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cowpea is an important grain legume crop of Africa, Latin America, and Southeast Asia. Leaf curl and golden mosaic diseases caused by Mungbean yellow mosaic India virus (MYMIV) have emerged as most devastating viral diseases of cowpea in Southeast Asia. In this study, we employed RNA interference (RNAi) strategy to control cowpea-infecting MYMIV. For this, we generated transgenic cowpea plants harbouring three different intron hairpin RNAi constructs, containing the AC2, AC4 and fusion of AC2 and AC4 (AC2+AC4) of seven cowpea-infecting begomoviruses. The T0 and T1 transgenic cowpea lines of all the three constructs accumulated transgene-specific siRNAs. Transgenic plants were further assayed up to T1 generations, for resistance to MYMIV using agro-infectious clones. Nearly 100% resistance against MYMIV infection was observed in transgenic lines, expressing AC2-hp and AC2+AC4-hp RNA, when compared with untransformed controls and plants transformed with empty vectors, which developed severe viral disease symptoms within 3 weeks. The AC4-hp RNA expressing lines displayed appearance of milder symptoms after 5 weeks of MYMIV-inoculation. Northern blots revealed a positive correlation between the level of transgene-specific siRNAs accumulation and virus resistance. The MYMIV-resistant transgenic lines accumulated nearly zero or very low titres of viral DNA. The transgenic cowpea plants had normal phenotype with no yield penalty in greenhouse conditions. This is the first demonstration of RNAi-derived resistance to MYMIV in cowpea.
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