ArticlePloS one2017
Modulation of benzylisoquinoline alkaloid biosynthesis by heterologous expression of CjWRKY1 in Eschscholzia californica cells.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 31 citations in OpenAlex.
- Whole transcriptome sequencing fromFrontiers in plant science · 2026Article
- Regulation of important natural products biosynthesis by WRKY transcription factors in plants.Journal of advanced research · 2025Review
- Network-based identification of hub transcription factors associated with benzylisoquinoline alkaloid biosynthesis inBiochemistry and biophysics reports · 2025Article
- Advances in the biosynthesis of naturally occurring benzylisoquinoline alkaloids.Frontiers in plant science · 2025Review
- Herbgenomics meets Papaveraceae: a promising -omics perspective on medicinal plant research.Briefings in functional genomics · 2024Review
- Integrated metabolite profiling and transcriptome analysis reveal candidate genes involved in the biosynthesis of benzylisoquinoline alkaloids inPlant biotechnology (Tokyo, Japan) · 2024Article
- Identification of P450 Candidates Associated with the Biosynthesis of Physalin-Class Compounds inInternational journal of molecular sciences · 2023Article
- Genomic profiling of WRKY transcription factors and functional analysis of CcWRKY7, CcWRKY29, and CcWRKY32 related to protoberberine alkaloids biosynthesis inFrontiers in genetics · 2023Article
- California poppy (Frontiers in plant science · 2023Review
- Interplay of transcription factors orchestrating the biosynthesis of plant alkaloids.3 Biotech · 2022Review
- Application of metabolic engineering to enhance the content of alkaloids in medicinal plants.Metabolic engineering communications · 2022Review
- An Update of the Sanguinarine and Benzophenanthridine Alkaloids' Biosynthesis and Their Applications.Molecules (Basel, Switzerland) · 2022Review
- Transcription Factors in Alkaloid Engineering.Biomolecules · 2021Review
- Transcriptomic Analyses Shed Light on Critical Genes Associated with Bibenzyl Biosynthesis inPlants (Basel, Switzerland) · 2021Article
- Genome-Wide Profiling ofFrontiers in plant science · 2021Article
- Genome-wide identification of AP2/ERF transcription factor-encoding genes in California poppy (Eschscholzia californica) and their expression profiles in response to methyl jasmonate.Scientific reports · 2020Article
- Regulatory Mechanisms of Benzylisoquinoline Alkaloid Biosynthesis in Coptis chinensis Under Light Quality Treatment.Physiologia plantarumArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription factors control many processes in plants and have high potentials to manipulate specialized metabolic pathways. Transcriptional regulation of the biosynthesis of monoterpenoid indole alkaloids (MIAs), nicotine alkaloids, and benzylisoquinoline alkaloids (BIAs) has been characterized using Catharanthus roseus, Nicotiana and Coptis plants. However, metabolic engineering in which specific transcription factors are used in alkaloid biosynthesis is limited. In this study, we characterized the effects of ectopic expression of CjWRKY1, which is a transcriptional activator with many targets in BIA biosynthesis in Coptis japonica (Ranunculaceae) and Eschscholzia californica (California poppy, Papaveraceae). Heterologous expression of CjWRKY1 in cultured California poppy cells induced increases in transcripts of several genes encoding BIA biosynthetic enzymes. Metabolite analyses indicated that the overexpression of the CjWRKY1 gene also induced increases in the accumulation of BIAs such as sanguinarine, chelerythrine, chelirubine, protopine, allocryptopine, and 10-hydroxychelerythrine in the culture medium. Previous characterization of EcbHLH1 and current results indicated that both transcription factors, WRKY1 and bHLH1, are substantially involved in the regulation of BIA biosynthesis. We discuss the function of CjWRKY1 in E. californica cells and its potential for metabolic engineering in BIA biosynthesis.
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