ArticleViruses2017
ABC Assay: Method Development and Application to Quantify the Role of Three DWV Master Variants in Overwinter Colony Losses of European Honey Bees.
Article in Viruses, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 89 citations in OpenAlex.
- Differential expression of neuronal function genes follows a tissue-specific temporal dynamic during Deformed Wing Virus infection in honeybees.Scientific reports · 2026Article
- Development of multiplex RT-PCR assays containing an internal amplification control for the detection of dicistro-, iflaviruses and CBPV in honey bees. Part 1 - assays design and optimization.Veterinary research communications · 2026Article
- Review
- Factors influencing the prevalence of acute bee paralysis virus in Apis mellifera and insights into its phylogenetic relationships.Virus genes · 2025Article
- Validation and comparison of EvaGreen- and TaqMan-based real-time qRT-PCR for diagnosis of the black queen cell virus in the honey bees.Veterinary research communications · 2025Article
- Genotype B of deformed wing virus and related recombinant viruses become dominant in European honey bee colonies.Scientific reports · 2025Article
- Honey bees bred for Varroa sensitive hygiene trait demonstrate resistance to chalkbrood disease.PloS one · 2025Article
- A novel antibody treatment reduces deformed wing virus loads in the western honey bee (mSphere · 2024Article
- Solenopsis richteri (Hymenoptera: Formicidae) alates infected with deformed wing virus display wing deformity with altered mobility.Die Naturwissenschaften · 2024Article
- Detection and characterization of Deformed Wing Virus (DWV) in apiaries with stationary and migratory management in the province of Entre Ríos, Argentina.Scientific reports · 2024Article
- Article
- Selection and Multiplexing of Reverse Transcription-Quantitative PCR Tests Targeting Relevant Honeybee Viral Pathogens.Microorganisms · 2024Article
- Quantitative microbiome profiling of honey bee (Apis mellifera) guts is predictive of winter colony loss in northern Virginia (USA).Scientific reports · 2024Article
- Seasonal Variability in the Prevalence of DWV Strains in Individual Colonies of European Honeybees in Hawaii.Insects · 2024Article
- A semi-automated and high-throughput approach for the detection of honey bee viruses in bee samples.PloS one · 2024Article
- Origins, diversity, and adaptive evolution of DWV in the honey bees of the Azores: the impact of the invasive miteVirus evolution · 2024Article
- No impact of hygienic behavior and viral coinfection on the development of European foulbrood in honey bee (Apis mellifera) colonies during blueberry pollination in Michigan.Journal of insect science (Online) · 2023Article
- Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus inInsects · 2023Article
- Influence of virus abundances in donor colonies and nurse hives on queens ofOpen veterinary journal · 2023Article
- Interaction between the VP2 protein of deformed wing virus and host snapin protein and its effect on viral replication.Frontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Deformed wing virus (DWV) is one of the most prevalent honey bee viral pathogens in the world. Typical of many RNA viruses, DWV is a quasi-species, which is comprised of a large number of different variants, currently consisting of three master variants: Type A, B, and C. Little is known about the impact of each variant or combinations of variants upon the biology of individual hosts. Therefore, we have developed a new set of master variant-specific DWV primers and a set of standards that allow for the quantification of each of the master variants. Competitive reverse transcriptase polymerase chain reaction (RT-PCR) experimental design confirms that each new DWV primer set is specific to the retrospective master variant. The sensitivity of the ABC assay is dependent on whether DNA or RNA is used as the template and whether other master variants are present in the sample. Comparison of the overall proportions of each master variant within a sample of known diversity, as confirmed by next-generation sequence (NGS) data, validates the efficiency of the ABC assay. The ABC assay was used on archived material from a Devon overwintering colony loss (OCL) 2006-2007 study; further implicating DWV type A and, for the first time, possibly C in the untimely collapse of honey bee colonies. Moreover, in this study DWV type B was not associated with OCL. The use of the ABC assay will allow researchers to quickly and cost effectively pre-screen for the presence of DWV master variants in honey bees.
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